Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis

Harm HogenEsch, Mario Sola, Timothy M. Stearns, Kathleen A. Silva, Victoria E. Kennedy, John P. Sundberg

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Angiogenesis is a common feature of pathological processes including wound healing, tumor formation, and chronic inflammation. Chronic inflammation can also be associated with dilation or proliferation of lymph vessels. We examined blood vessels and lymphatics and the expression of pro- and anti-angiogenic genes in the skin of SHARPIN-deficient mice which spontaneously develop a chronic proliferative dermatitis (cpdm). The number of blood vessels in the dermis of cpdm mice increased with age as the inflammation progressed. Lymphatics identified by labeling for LYVE1 and podoplanin were moderately dilated, but they were not increased in number. The expression of proangiogenic Vegfa, Flt1 and anti-angiogenic Sema3a mRNA was increased. VEGFA was primarily localized in keratinocytes of cpdm skin. There was also increased expression of Ece1 and Pdpn mRNA. Podoplanin was restricted to lymphatic endothelial cells in normal skin, but fibroblasts in cpdm skin also reacted with anti-podoplanin antibodies indicating that they were activated. The expression of other angiogenic and lymphangiogenic factors was not altered or decreased. These results indicate that cpdm mice may be a useful model to study the pathogenesis of angiogenesis in chronic inflammation.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalExperimental and Molecular Pathology
Volume101
Issue number3
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Keywords

  • Angiogenesis
  • Dermatitis
  • Lymphangiogenesis
  • Podoplanin
  • VEGF

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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