Annexin-2 is a regulator of stromal cell-derived factor-1/CXCL12 function in the hematopoietic stem cell endosteal niche

Younghun Jung, Yusuke Shiozawa, Jingcheng Wang, Lalit R. Patel, Aaron M. Havens, Junhui Song, Paul H. Krebsbach, G. David Roodman, Russell S. Taichman

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: Previously, we reported that annexin-2 (anxa2) plays an important role in hematopoietic stem cell (HSC) localization to the endosteal/osteoblastic marrow niche. This study explored the role that annexin-2 plays in presenting stromal cell-derived factor-1 (or CXCL12) to HSCs. Materials and Methods: Competitive long-term bone marrow transplant assays were used to determine if HSC engraftment is altered in annexin-2-deficient animals. Colony-forming cell assays, CXCL12 enzyme-linked immunosorbent assay, and real-time reverse transcription polymerase chain reaction analyses were used to determine stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Immunohistochemistry, immunoprecipitation, binding assays, and chemotactic assays were employed to determine if annexin-2 is associated with CXCL12. Degradation assays were also used to determine if annexin-2 and CXCL12 protect each other from proteolytic degradation. Results: Anxa2-/- animals had fewer HSCs in their marrow, and the HSCs in anxa2-/- animals express less CXCR4 and CXCR7, suggesting a cell intrinsic defect. Transplantation studies of wild-type marrow into anxa2-/- animals demonstrated a cell-extrinsic defect in the anxa2-/- animals. CXCL12 binds directly to annexin-2, and this interaction facilitates presentation of CXCL12 to HSCs. Yet the binding of CXCL12 to annexin-2 did not protect CXCL12 from proteolytic cleavage after stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Conclusions: These results suggest that annexin-2 serves as an anchor for CXCL12 to help in the localization of HSCs to the niche.

Original languageEnglish (US)
JournalExperimental Hematology
Volume39
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Stem Cell Niche
Annexins
Chemokine CXCL12
Hematopoietic Stem Cells
Stem Cells
Bone Marrow
Granulocyte Colony-Stimulating Factor
Immunoprecipitation
Reverse Transcription
Transplantation
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Transplants
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology

Cite this

Annexin-2 is a regulator of stromal cell-derived factor-1/CXCL12 function in the hematopoietic stem cell endosteal niche. / Jung, Younghun; Shiozawa, Yusuke; Wang, Jingcheng; Patel, Lalit R.; Havens, Aaron M.; Song, Junhui; Krebsbach, Paul H.; Roodman, G. David; Taichman, Russell S.

In: Experimental Hematology, Vol. 39, No. 2, 02.2011.

Research output: Contribution to journalArticle

Jung, Younghun ; Shiozawa, Yusuke ; Wang, Jingcheng ; Patel, Lalit R. ; Havens, Aaron M. ; Song, Junhui ; Krebsbach, Paul H. ; Roodman, G. David ; Taichman, Russell S. / Annexin-2 is a regulator of stromal cell-derived factor-1/CXCL12 function in the hematopoietic stem cell endosteal niche. In: Experimental Hematology. 2011 ; Vol. 39, No. 2.
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abstract = "Objective: Previously, we reported that annexin-2 (anxa2) plays an important role in hematopoietic stem cell (HSC) localization to the endosteal/osteoblastic marrow niche. This study explored the role that annexin-2 plays in presenting stromal cell-derived factor-1 (or CXCL12) to HSCs. Materials and Methods: Competitive long-term bone marrow transplant assays were used to determine if HSC engraftment is altered in annexin-2-deficient animals. Colony-forming cell assays, CXCL12 enzyme-linked immunosorbent assay, and real-time reverse transcription polymerase chain reaction analyses were used to determine stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Immunohistochemistry, immunoprecipitation, binding assays, and chemotactic assays were employed to determine if annexin-2 is associated with CXCL12. Degradation assays were also used to determine if annexin-2 and CXCL12 protect each other from proteolytic degradation. Results: Anxa2-/- animals had fewer HSCs in their marrow, and the HSCs in anxa2-/- animals express less CXCR4 and CXCR7, suggesting a cell intrinsic defect. Transplantation studies of wild-type marrow into anxa2-/- animals demonstrated a cell-extrinsic defect in the anxa2-/- animals. CXCL12 binds directly to annexin-2, and this interaction facilitates presentation of CXCL12 to HSCs. Yet the binding of CXCL12 to annexin-2 did not protect CXCL12 from proteolytic cleavage after stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Conclusions: These results suggest that annexin-2 serves as an anchor for CXCL12 to help in the localization of HSCs to the niche.",
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AU - Jung, Younghun

AU - Shiozawa, Yusuke

AU - Wang, Jingcheng

AU - Patel, Lalit R.

AU - Havens, Aaron M.

AU - Song, Junhui

AU - Krebsbach, Paul H.

AU - Roodman, G. David

AU - Taichman, Russell S.

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AB - Objective: Previously, we reported that annexin-2 (anxa2) plays an important role in hematopoietic stem cell (HSC) localization to the endosteal/osteoblastic marrow niche. This study explored the role that annexin-2 plays in presenting stromal cell-derived factor-1 (or CXCL12) to HSCs. Materials and Methods: Competitive long-term bone marrow transplant assays were used to determine if HSC engraftment is altered in annexin-2-deficient animals. Colony-forming cell assays, CXCL12 enzyme-linked immunosorbent assay, and real-time reverse transcription polymerase chain reaction analyses were used to determine stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Immunohistochemistry, immunoprecipitation, binding assays, and chemotactic assays were employed to determine if annexin-2 is associated with CXCL12. Degradation assays were also used to determine if annexin-2 and CXCL12 protect each other from proteolytic degradation. Results: Anxa2-/- animals had fewer HSCs in their marrow, and the HSCs in anxa2-/- animals express less CXCR4 and CXCR7, suggesting a cell intrinsic defect. Transplantation studies of wild-type marrow into anxa2-/- animals demonstrated a cell-extrinsic defect in the anxa2-/- animals. CXCL12 binds directly to annexin-2, and this interaction facilitates presentation of CXCL12 to HSCs. Yet the binding of CXCL12 to annexin-2 did not protect CXCL12 from proteolytic cleavage after stem or progenitor cell mobilization by granulocyte colony-stimulating factor. Conclusions: These results suggest that annexin-2 serves as an anchor for CXCL12 to help in the localization of HSCs to the niche.

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