Antibacterial TAP-mimic electrospun polymer scaffold

effects on P. gingivalis-infected dentin biofilm

Maria Tereza P Albuquerque, Joshua D. Evans, Richard Gregory, Marcia C. Valera, Marco C. Bottino

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods: Dentin specimens (4 × 4 × 1 mm3) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results: Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p 10 CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions: The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance: Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalClinical Oral Investigations
Volume20
Issue number2
DOIs
StatePublished - Mar 1 2016

Fingerprint

Dentin
Biofilms
Ointments
Polymers
Anti-Bacterial Agents
Porphyromonas gingivalis
Stem Cells
Polydioxanone
Minocycline
Endodontics
Disinfection
Metronidazole
Ciprofloxacin
Sodium Chloride
Electron Scanning Microscopy
Canidae
Bacteria

Keywords

  • Antibiotic
  • Disinfection
  • Electrospinning
  • Endodontics
  • Nanofibers
  • Scaffold

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antibacterial TAP-mimic electrospun polymer scaffold : effects on P. gingivalis-infected dentin biofilm. / Albuquerque, Maria Tereza P; Evans, Joshua D.; Gregory, Richard; Valera, Marcia C.; Bottino, Marco C.

In: Clinical Oral Investigations, Vol. 20, No. 2, 01.03.2016, p. 387-393.

Research output: Contribution to journalArticle

Albuquerque, Maria Tereza P ; Evans, Joshua D. ; Gregory, Richard ; Valera, Marcia C. ; Bottino, Marco C. / Antibacterial TAP-mimic electrospun polymer scaffold : effects on P. gingivalis-infected dentin biofilm. In: Clinical Oral Investigations. 2016 ; Vol. 20, No. 2. pp. 387-393.
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