Antibodies from women immunized with Gardasil® cross-neutralize HPV 45 pseudovirions

Judith F. Smith, Michelle Brownlow, Martha Brown, Rose Kowalski, Mark T. Esser, Wanda Ruiz, Eliav Barr, Darron R. Brown, Janine T. Bryan

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

Human papillomavirus (HPV) types 6, 11, 16 and 18 L1 virus-like particles (VLPs) have been used to generate the prophylactic quadrivalent vaccine, Gardasil®. There is a high degree of L1 homology between HPV types and it is likely that there is a substantial degree of surface exposed viral epitope similarity. An investigation of vaccine-induced antibody binding and neutralization was undertaken focusing on A7 species members, HPV 18 and 45. Polyclonal sera from Gardasil® recipients and from HPV 18 L1 VLP recipients were evaluated. Vaccine-induced antibodies were found to cross-neutralize HPV 45 pseudovirions (PsV) in vitro. Examination of a panel of monoclonal antibodies made against L1 VLPs revealed the presence of conformational, neutralizing epitopes on the surface of VLPs that may be shared between HPV 18 and HPV 45. These data demonstrate that Gardasil(r) immunization induces antibodies capable of neutralizing HPV 18 PsV and HPV 45 PsV in vitro.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalHuman Vaccines
Volume3
Issue number4
DOIs
StatePublished - Jan 1 2007

Keywords

  • Cross-neutralization
  • Gardasil®
  • HPV 45
  • HPV vaccine
  • Immune response

ASJC Scopus subject areas

  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Smith, J. F., Brownlow, M., Brown, M., Kowalski, R., Esser, M. T., Ruiz, W., Barr, E., Brown, D. R., & Bryan, J. T. (2007). Antibodies from women immunized with Gardasil® cross-neutralize HPV 45 pseudovirions. Human Vaccines, 3(4), 109-115. https://doi.org/10.4161/hv.3.4.4058