Antibody to Pneumocystis carinii protects rats and mice from developing pneumonia

Marilyn S. Bartlett, William C. Angus, Margaret M. Shaw, Pamela J. Durant, Chao Hung Lee, Juan M. Pascale, James W. Smith

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Well-proven mouse and rat models were used to show that polyclonal antisera to Pneumocystis carinii protect against P. carinii pneumonia. Antibodies were obtained from animals that were allowed to recover from severe P. carinii pneumonia after immunosuppression had been stopped and which then were given a booster injection of P. carinii from the same animal species. Mice immunosuppressed with corticosteroids or antibodies to L3T4+ lymphocytes (which are comparable to CD4 cells of humans) and transtracheally inoculated with mouse P. carinii did not develop P. carinii pneumonia if they were passively immunized with antiserum, while mice immunosuppressed and inoculated by identical procedures but not given antibodies developed severe infections. Rats immunosuppressed with corticosteroids and inoculated with rat P. carinii had less severe infections if they were given rat anti-P. carinii antisera. The polyclonal antisera developed in mice provided greater protection for the mice than the polyclonal rat antisera did for the rats; however, the potencies and compositions of the antisera were not quantitated and probably differed. Since both rats and mice can be protected from P. carinii infections with polyclonal antisera, it may be possible to develop vaccines that will elicit protective antibodies in humans.

Original languageEnglish (US)
Pages (from-to)74-77
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume5
Issue number1
StatePublished - Feb 16 1998

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Bartlett, M. S., Angus, W. C., Shaw, M. M., Durant, P. J., Lee, C. H., Pascale, J. M., & Smith, J. W. (1998). Antibody to Pneumocystis carinii protects rats and mice from developing pneumonia. Clinical and Diagnostic Laboratory Immunology, 5(1), 74-77.