Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies

Byron Batteiger, W. J. Newhall, P. Terho, C. E. Wilde, R. B. Jones

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Abstract

We prepared monoclonal antibodies against prototype strains of the 15 serovars of Chlamydia trachomatis and identified a subset of reagents that reacted with the major outer membrane protein(s) (MOMPs) of one or more serovars. We then determined the specificities of these anti-MOMP monoclonal antibodies by radioimmunoassay and immunoblot assays against the 15 serovars of C. trachomatis and a C. psittaci strain. We identified 14 different anti-MOMP antibody specificities, including serovar-, several orders of subspecies-, and species-specific determinants. In addition, one antibody reacted with all C. trachomatis serovars and a C. psittaci strain, indicating the presence of a genus-specific epitope on MOMP. Many of the cross-reactions of the subspecies-specific antibodies were similar to those previously reported by use of the microimmunofluorescence technique. We also observed a number of cross-reactions that were unexpected but consistent with data derived by the microimmunofluorescence test. All antibodies, except the genus-specific antibodies, reacted with whole elementary bodies in a radioimmunoassay, suggesting surface exposure of the epitopes. These data confirm and extend previous observations that MOMPs among C. trachomatis serovars are antigenically complex and diverse. In addition, these data indicate that the cross-reaction patterns of some monoclonal antibodies directed against MOMP are similar to those detected by the microimmunofluorescence test and are consistent with the hypothesis that such determinants are contained within MOMPs.

Original languageEnglish
Pages (from-to)530-533
Number of pages4
JournalInfection and Immunity
Volume53
Issue number3
StatePublished - 1986

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Chlamydia trachomatis
Monoclonal Antibodies
Cross Reactions
Antibodies
Radioimmunoassay
Epitopes
Antibody Specificity
Serogroup
Chlamydia trachomatis omp1 protein
Anti-Idiotypic Antibodies
Membrane Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies. / Batteiger, Byron; Newhall, W. J.; Terho, P.; Wilde, C. E.; Jones, R. B.

In: Infection and Immunity, Vol. 53, No. 3, 1986, p. 530-533.

Research output: Contribution to journalArticle

Batteiger, Byron ; Newhall, W. J. ; Terho, P. ; Wilde, C. E. ; Jones, R. B. / Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies. In: Infection and Immunity. 1986 ; Vol. 53, No. 3. pp. 530-533.
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