Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles

J. T. Stapleton, V. Raina, P. L. Winokur, K. Walters, D. Klinzman, E. Rosen, J. H. McLinden

Research output: Contribution to journalComment/debate

34 Scopus citations

Abstract

Hepatitis A virus (HAV) has an immunodominant neutralization antigenic site. By using a panel of monoclonal antibodies targeted against the HAV neutralization antigenic site, it was shown that three epitopes within this site are present on 14S subunits (pentamers of the structural unit). In contrast, two other epitopes within this site are formed upon assembly of 14S subunits into capsids. Thus, the epitopes recognized by these two monoclonal antibodies are formed either by a conformational change in the antigenic site or by the juxtaposition of epitope fragments present on different 14S subunits during assembly of 14S into 70S particles. Both 14S and 70S particles elicited HAV-neutralizing antibodies in mice; thus, these particles may be useful for HAV vaccine development.

Original languageEnglish (US)
Pages (from-to)1080-1085
Number of pages6
JournalJournal of virology
Volume67
Issue number2
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Stapleton, J. T., Raina, V., Winokur, P. L., Walters, K., Klinzman, D., Rosen, E., & McLinden, J. H. (1993). Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. Journal of virology, 67(2), 1080-1085.