Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles

Jack T. Stapleton, Vijay Raina, Patricia L. Winokur, Kathy Walters, Donna Klinzman, Elliot Rosen, James H. McLinden

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Hepatitis A virus (HAV) has an immunodominant neutralization antigenic site. By using a panel of monoclonal antibodies targeted against the HAV neutralization antigenic site, it was shown that three epitopes within this site are present on 14S subunits (pentamers of the structural unit). In contrast, two other epitopes within this site are formed upon assembly of 14S subunits into capsids. Thus, the epitopes recognized by these two monoclonal antibodies are formed either by a conformational change in the antigenic site or by the juxtaposition of epitope fragments present on different 14S subunits during assembly of 14S into 70S particles. Both 14S and 70S particles elicited HAV-neutralizing antibodies in mice; thus, these particles may be useful for HAV vaccine development.

Original languageEnglish (US)
Pages (from-to)1080-1085
Number of pages6
JournalJournal of Virology
Volume67
Issue number2
StatePublished - Feb 1993
Externally publishedYes

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Hepatitis A virus
epitopes
Epitopes
monoclonal antibodies
Monoclonal Antibodies
Hepatitis A Vaccines
Hepatitis A Antibodies
capsid
Capsid
vaccine development
neutralization tests
Neutralizing Antibodies
neutralizing antibodies
neutralization
mice

ASJC Scopus subject areas

  • Immunology

Cite this

Stapleton, J. T., Raina, V., Winokur, P. L., Walters, K., Klinzman, D., Rosen, E., & McLinden, J. H. (1993). Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. Journal of Virology, 67(2), 1080-1085.

Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. / Stapleton, Jack T.; Raina, Vijay; Winokur, Patricia L.; Walters, Kathy; Klinzman, Donna; Rosen, Elliot; McLinden, James H.

In: Journal of Virology, Vol. 67, No. 2, 02.1993, p. 1080-1085.

Research output: Contribution to journalArticle

Stapleton, JT, Raina, V, Winokur, PL, Walters, K, Klinzman, D, Rosen, E & McLinden, JH 1993, 'Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles', Journal of Virology, vol. 67, no. 2, pp. 1080-1085.
Stapleton JT, Raina V, Winokur PL, Walters K, Klinzman D, Rosen E et al. Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. Journal of Virology. 1993 Feb;67(2):1080-1085.
Stapleton, Jack T. ; Raina, Vijay ; Winokur, Patricia L. ; Walters, Kathy ; Klinzman, Donna ; Rosen, Elliot ; McLinden, James H. / Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. In: Journal of Virology. 1993 ; Vol. 67, No. 2. pp. 1080-1085.
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