Antioxidants reverse age-related collateral growth impairment

Steven Miller, Brian J. Coppinger, Xiaosun Zhou, Joseph L. Unthank

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aging is a major risk factor for the development of cardiovascular diseases, including arterial occlusive disease. Oxidant stress increases with age, and may be a significant factor contributing to vascular dysfunction and disease. We have shown that aging and hypertension impair collateral growth, the natural compensatory response to arterial occlusive disease, and that antioxidants restore collateral growth in young hypertensive rats. The aim of this study was to test the hypothesis that oxidant stress mediates collateral growth impairment in nondiseased, aged rats. Ileal arteries were induced to become collaterals via ligation of adjacent arteries. Growth was assessed at 7 days by repeated in vivo measurements and comparison to same-animal control arteries. Collateral diameter enlargement did not occur in aged rats, but luminal expansion was stimulated by pretreatment with tempol. Co-administration of L-NAME with tempol prevented tempol-mediated collateral development. Expression of p22phox mRNA was increased in aged versus young rat arteries, suggesting NAD(P)H oxidase as a source of reactive oxygen species. Treatment with apocynin increased collateral growth capacity, whether administered prior to, or 7 days following, arterial ligation. The results suggest that antioxidant treatment may be useful in promoting collateral growth to compensate for age-related arterial occlusive disease.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalJournal of Vascular Research
Volume47
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Antioxidants
Arterial Occlusive Diseases
Growth
Arteries
Oxidants
Ligation
NADPH Oxidase
NG-Nitroarginine Methyl Ester
Vascular Diseases
Reactive Oxygen Species
Cardiovascular Diseases
Hypertension
Messenger RNA
tempol

Keywords

  • Apocynin
  • Arteriogenesis
  • Nitric oxide
  • Tempol

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Antioxidants reverse age-related collateral growth impairment. / Miller, Steven; Coppinger, Brian J.; Zhou, Xiaosun; Unthank, Joseph L.

In: Journal of Vascular Research, Vol. 47, No. 2, 02.2010, p. 108-114.

Research output: Contribution to journalArticle

Miller, Steven ; Coppinger, Brian J. ; Zhou, Xiaosun ; Unthank, Joseph L. / Antioxidants reverse age-related collateral growth impairment. In: Journal of Vascular Research. 2010 ; Vol. 47, No. 2. pp. 108-114.
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