Antiretroviral Therapy Normalizes Autoantibody Profile of HIV Patients by Decreasing CD33+ CD11b+ HLA-DR+ Cells

Zhefeng Meng, Ling Du, Ningjie Hu, Daniel Byrd, Tohti Amet, Mona Desai, Nicole Shepherd, Jie Lan, Renzhi Han, Andy Yu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Autoimmune manifestations are common in human immunodeficiency virus (HIV) patients. However, the autoantibody spectrum associated with HIV infection and the impact of antiretroviral therapy (ART) remains to be determined. The plasma autoantibody spectrum for HIV patients was characterized by protein microarrays containing 83 autoantigens and confirmed by enzyme-linked immunosorbent assay (ELISA). Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) were analyzed by flow cytometry and their effects on autoantibodies production were determined by B cell ELISpot. Higher levels of autoantibody and higher prevalence of elevated autoantibodies were observed in ART-naive HIV patients compared to healthy subjects and HIV patients on ART. The highest frequency of CD33+ CD11b+ HLA-DR+ cells was observed in ART-naive HIV patients and was associated with the quantity of elevated autoantibodies. In addition, CD33+ CD11b + HLA-DR+ cells other than Tregs or MDSCs boost the B cell response in a dose-dependent manner by in vitro assay. In summary, HIV infection leads to elevation of autoantibodies while ART suppresses the autoimmune manifestation by decreasing CD33+ CD11b+ HLA-DR+ cells in vivo. The roles of CD33+ CD11b+ HLA-DR+ cells on disease progression in HIV patients needs further assessment.

Original languageEnglish (US)
Article numbere3285
JournalMedicine (United States)
Volume95
Issue number15
DOIs
StatePublished - Apr 1 2016

Fingerprint

HLA-DR Antigens
Autoantibodies
HIV
Virus Diseases
Therapeutics
B-Lymphocytes
Protein Array Analysis
Needs Assessment
Autoantigens
Regulatory T-Lymphocytes
Disease Progression
Healthy Volunteers
Flow Cytometry
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Antiretroviral Therapy Normalizes Autoantibody Profile of HIV Patients by Decreasing CD33+ CD11b+ HLA-DR+ Cells. / Meng, Zhefeng; Du, Ling; Hu, Ningjie; Byrd, Daniel; Amet, Tohti; Desai, Mona; Shepherd, Nicole; Lan, Jie; Han, Renzhi; Yu, Andy.

In: Medicine (United States), Vol. 95, No. 15, e3285, 01.04.2016.

Research output: Contribution to journalArticle

Meng, Zhefeng ; Du, Ling ; Hu, Ningjie ; Byrd, Daniel ; Amet, Tohti ; Desai, Mona ; Shepherd, Nicole ; Lan, Jie ; Han, Renzhi ; Yu, Andy. / Antiretroviral Therapy Normalizes Autoantibody Profile of HIV Patients by Decreasing CD33+ CD11b+ HLA-DR+ Cells. In: Medicine (United States). 2016 ; Vol. 95, No. 15.
@article{f64c41b78099495690046435bd2bf86f,
title = "Antiretroviral Therapy Normalizes Autoantibody Profile of HIV Patients by Decreasing CD33+ CD11b+ HLA-DR+ Cells",
abstract = "Autoimmune manifestations are common in human immunodeficiency virus (HIV) patients. However, the autoantibody spectrum associated with HIV infection and the impact of antiretroviral therapy (ART) remains to be determined. The plasma autoantibody spectrum for HIV patients was characterized by protein microarrays containing 83 autoantigens and confirmed by enzyme-linked immunosorbent assay (ELISA). Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) were analyzed by flow cytometry and their effects on autoantibodies production were determined by B cell ELISpot. Higher levels of autoantibody and higher prevalence of elevated autoantibodies were observed in ART-naive HIV patients compared to healthy subjects and HIV patients on ART. The highest frequency of CD33+ CD11b+ HLA-DR+ cells was observed in ART-naive HIV patients and was associated with the quantity of elevated autoantibodies. In addition, CD33+ CD11b + HLA-DR+ cells other than Tregs or MDSCs boost the B cell response in a dose-dependent manner by in vitro assay. In summary, HIV infection leads to elevation of autoantibodies while ART suppresses the autoimmune manifestation by decreasing CD33+ CD11b+ HLA-DR+ cells in vivo. The roles of CD33+ CD11b+ HLA-DR+ cells on disease progression in HIV patients needs further assessment.",
author = "Zhefeng Meng and Ling Du and Ningjie Hu and Daniel Byrd and Tohti Amet and Mona Desai and Nicole Shepherd and Jie Lan and Renzhi Han and Andy Yu",
year = "2016",
month = "4",
day = "1",
doi = "10.1097/MD.0000000000003285",
language = "English (US)",
volume = "95",
journal = "Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries",
issn = "0025-7974",
publisher = "Lippincott Williams and Wilkins",
number = "15",

}

TY - JOUR

T1 - Antiretroviral Therapy Normalizes Autoantibody Profile of HIV Patients by Decreasing CD33+ CD11b+ HLA-DR+ Cells

AU - Meng, Zhefeng

AU - Du, Ling

AU - Hu, Ningjie

AU - Byrd, Daniel

AU - Amet, Tohti

AU - Desai, Mona

AU - Shepherd, Nicole

AU - Lan, Jie

AU - Han, Renzhi

AU - Yu, Andy

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Autoimmune manifestations are common in human immunodeficiency virus (HIV) patients. However, the autoantibody spectrum associated with HIV infection and the impact of antiretroviral therapy (ART) remains to be determined. The plasma autoantibody spectrum for HIV patients was characterized by protein microarrays containing 83 autoantigens and confirmed by enzyme-linked immunosorbent assay (ELISA). Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) were analyzed by flow cytometry and their effects on autoantibodies production were determined by B cell ELISpot. Higher levels of autoantibody and higher prevalence of elevated autoantibodies were observed in ART-naive HIV patients compared to healthy subjects and HIV patients on ART. The highest frequency of CD33+ CD11b+ HLA-DR+ cells was observed in ART-naive HIV patients and was associated with the quantity of elevated autoantibodies. In addition, CD33+ CD11b + HLA-DR+ cells other than Tregs or MDSCs boost the B cell response in a dose-dependent manner by in vitro assay. In summary, HIV infection leads to elevation of autoantibodies while ART suppresses the autoimmune manifestation by decreasing CD33+ CD11b+ HLA-DR+ cells in vivo. The roles of CD33+ CD11b+ HLA-DR+ cells on disease progression in HIV patients needs further assessment.

AB - Autoimmune manifestations are common in human immunodeficiency virus (HIV) patients. However, the autoantibody spectrum associated with HIV infection and the impact of antiretroviral therapy (ART) remains to be determined. The plasma autoantibody spectrum for HIV patients was characterized by protein microarrays containing 83 autoantigens and confirmed by enzyme-linked immunosorbent assay (ELISA). Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) were analyzed by flow cytometry and their effects on autoantibodies production were determined by B cell ELISpot. Higher levels of autoantibody and higher prevalence of elevated autoantibodies were observed in ART-naive HIV patients compared to healthy subjects and HIV patients on ART. The highest frequency of CD33+ CD11b+ HLA-DR+ cells was observed in ART-naive HIV patients and was associated with the quantity of elevated autoantibodies. In addition, CD33+ CD11b + HLA-DR+ cells other than Tregs or MDSCs boost the B cell response in a dose-dependent manner by in vitro assay. In summary, HIV infection leads to elevation of autoantibodies while ART suppresses the autoimmune manifestation by decreasing CD33+ CD11b+ HLA-DR+ cells in vivo. The roles of CD33+ CD11b+ HLA-DR+ cells on disease progression in HIV patients needs further assessment.

UR - http://www.scopus.com/inward/record.url?scp=84964624184&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964624184&partnerID=8YFLogxK

U2 - 10.1097/MD.0000000000003285

DO - 10.1097/MD.0000000000003285

M3 - Article

VL - 95

JO - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries

JF - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries

SN - 0025-7974

IS - 15

M1 - e3285

ER -