Apoptosis in regenerating and denervated, nonregenerating urodele forelimbs

Anthony Mescher, Gary White, James J. Brokaw

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Denervated limbs of larval salamanders fail to regenerate if amputated and, unlike adult limbs, undergo regression. The cellular basis of the tissue loss is poorly understood. We used TUNEL staining of larval axolotl limbs fixed and sectioned at intervals after bilateral amputation and unilateral denervation to investigate the role of apoptosis during normal limb regeneration and denervated limb regression. In the first week after amputation a small percentage of apoptotic cells was found in both innervated and denervated limbs. During the second week the apoptotic index remained low in the mitotically active mesenchymal cells of the regenerating limbs, but increased twofold in the nondividing, dedifferentiated cells of the regressing limbs. TUNEL-positive cells resembling apoptotic bodies were restricted primarily to the dedifferentiated area beneath the wound epithelium, but were also present within the wound epithelium itself. Macrophages were identified immunohistochemically and were also found in increased numbers in distal areas of the denervated regressing limbs. The results suggest a role for apoptosis in the early phase of normal regeneration and indicate that denervated limb regression involves an increased rate of apoptosis and removal of apoptotic bodies by macrophages and the wound epithelium.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalWound Repair and Regeneration
Volume8
Issue number2
DOIs
StatePublished - Mar 2000

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Forelimb
Extremities
Apoptosis
Epithelium
In Situ Nick-End Labeling
Amputation
Regeneration
Wounds and Injuries
Macrophages
Ambystoma mexicanum
Urodela
Denervation
Staining and Labeling

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Apoptosis in regenerating and denervated, nonregenerating urodele forelimbs. / Mescher, Anthony; White, Gary; Brokaw, James J.

In: Wound Repair and Regeneration, Vol. 8, No. 2, 03.2000, p. 110-116.

Research output: Contribution to journalArticle

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