Apoptosis in spermatocytic and usual seminomas: A light microscopic and immunohistochemical study

Elena F. Bishop, Sunil Badve, Akira Morimiya, Rashmil Saxena, Thomas Ulbright

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Despite its alarming appearance, spermatocytic seminoma virtually never metastasizes. We hypothesized that this paradox may at least be partially related to increased apoptosis compared to metastasizing germ cell tumors since high expression of proapoptotic factors correlates with indolent behavior in other tumor systems, notably CD30-positive cutaneous lymphoma, another neoplasm where phenotype and behavior do not match. We therefore compared apoptosis and apoptotic regulators in 17 spermatocytic seminomas (2 with sarcoma) and 18 usual seminomas by light microscopy and using immunostains for caspase-3, p53, bcl-2, bcl-xL, FADD, FAS and survivin. We found significantly greater numbers of apoptotic cells and activated caspase-3-positive cells in spermatocytic seminoma compared to usual seminoma (P<0.01). There was over a 10-fold range in apoptotic cells in usual seminoma but only a 4-fold variation in spermatocytic seminoma. Spermatocytic seminoma had decreased p53 expression compared to usual seminoma, with marked variation in bcl-2 expression and increased FADD. The two sarcomas in spermatocytic seminoma, however, showed decreased apoptosis and caspase-3 reactivity, with upregulation of p53 and bcl-2 and decreased FADD expression. We conclude that apoptosis, caspase-3 and FADD expression are increased in spermatocytic seminoma compared to usual seminoma. Apoptotic parameters are decreased in sarcomatous transformation of spermatocytic seminoma. The increased apoptosis of spermatocytic seminoma, possibly mediated by FAS independent activation of the death receptor pathway, may provide some insight into its excellent prognosis. The variation in apoptosis of usual seminomas merits investigation as a prognostic parameter.

Original languageEnglish
Pages (from-to)1036-1044
Number of pages9
JournalModern Pathology
Volume20
Issue number10
DOIs
StatePublished - Oct 2007

Fingerprint

Seminoma
Apoptosis
Light
Caspase 3
Sarcoma
Death Domain Receptors
Germ Cell and Embryonal Neoplasms

Keywords

  • Apoptosis
  • Proapoptotic factors
  • Seminoma
  • Spermatocytic seminoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Apoptosis in spermatocytic and usual seminomas : A light microscopic and immunohistochemical study. / Bishop, Elena F.; Badve, Sunil; Morimiya, Akira; Saxena, Rashmil; Ulbright, Thomas.

In: Modern Pathology, Vol. 20, No. 10, 10.2007, p. 1036-1044.

Research output: Contribution to journalArticle

Bishop, Elena F. ; Badve, Sunil ; Morimiya, Akira ; Saxena, Rashmil ; Ulbright, Thomas. / Apoptosis in spermatocytic and usual seminomas : A light microscopic and immunohistochemical study. In: Modern Pathology. 2007 ; Vol. 20, No. 10. pp. 1036-1044.
@article{afeeb23db27847ea91561a91abebd4b0,
title = "Apoptosis in spermatocytic and usual seminomas: A light microscopic and immunohistochemical study",
abstract = "Despite its alarming appearance, spermatocytic seminoma virtually never metastasizes. We hypothesized that this paradox may at least be partially related to increased apoptosis compared to metastasizing germ cell tumors since high expression of proapoptotic factors correlates with indolent behavior in other tumor systems, notably CD30-positive cutaneous lymphoma, another neoplasm where phenotype and behavior do not match. We therefore compared apoptosis and apoptotic regulators in 17 spermatocytic seminomas (2 with sarcoma) and 18 usual seminomas by light microscopy and using immunostains for caspase-3, p53, bcl-2, bcl-xL, FADD, FAS and survivin. We found significantly greater numbers of apoptotic cells and activated caspase-3-positive cells in spermatocytic seminoma compared to usual seminoma (P<0.01). There was over a 10-fold range in apoptotic cells in usual seminoma but only a 4-fold variation in spermatocytic seminoma. Spermatocytic seminoma had decreased p53 expression compared to usual seminoma, with marked variation in bcl-2 expression and increased FADD. The two sarcomas in spermatocytic seminoma, however, showed decreased apoptosis and caspase-3 reactivity, with upregulation of p53 and bcl-2 and decreased FADD expression. We conclude that apoptosis, caspase-3 and FADD expression are increased in spermatocytic seminoma compared to usual seminoma. Apoptotic parameters are decreased in sarcomatous transformation of spermatocytic seminoma. The increased apoptosis of spermatocytic seminoma, possibly mediated by FAS independent activation of the death receptor pathway, may provide some insight into its excellent prognosis. The variation in apoptosis of usual seminomas merits investigation as a prognostic parameter.",
keywords = "Apoptosis, Proapoptotic factors, Seminoma, Spermatocytic seminoma",
author = "Bishop, {Elena F.} and Sunil Badve and Akira Morimiya and Rashmil Saxena and Thomas Ulbright",
year = "2007",
month = "10",
doi = "10.1038/modpathol.3800933",
language = "English",
volume = "20",
pages = "1036--1044",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Apoptosis in spermatocytic and usual seminomas

T2 - A light microscopic and immunohistochemical study

AU - Bishop, Elena F.

AU - Badve, Sunil

AU - Morimiya, Akira

AU - Saxena, Rashmil

AU - Ulbright, Thomas

PY - 2007/10

Y1 - 2007/10

N2 - Despite its alarming appearance, spermatocytic seminoma virtually never metastasizes. We hypothesized that this paradox may at least be partially related to increased apoptosis compared to metastasizing germ cell tumors since high expression of proapoptotic factors correlates with indolent behavior in other tumor systems, notably CD30-positive cutaneous lymphoma, another neoplasm where phenotype and behavior do not match. We therefore compared apoptosis and apoptotic regulators in 17 spermatocytic seminomas (2 with sarcoma) and 18 usual seminomas by light microscopy and using immunostains for caspase-3, p53, bcl-2, bcl-xL, FADD, FAS and survivin. We found significantly greater numbers of apoptotic cells and activated caspase-3-positive cells in spermatocytic seminoma compared to usual seminoma (P<0.01). There was over a 10-fold range in apoptotic cells in usual seminoma but only a 4-fold variation in spermatocytic seminoma. Spermatocytic seminoma had decreased p53 expression compared to usual seminoma, with marked variation in bcl-2 expression and increased FADD. The two sarcomas in spermatocytic seminoma, however, showed decreased apoptosis and caspase-3 reactivity, with upregulation of p53 and bcl-2 and decreased FADD expression. We conclude that apoptosis, caspase-3 and FADD expression are increased in spermatocytic seminoma compared to usual seminoma. Apoptotic parameters are decreased in sarcomatous transformation of spermatocytic seminoma. The increased apoptosis of spermatocytic seminoma, possibly mediated by FAS independent activation of the death receptor pathway, may provide some insight into its excellent prognosis. The variation in apoptosis of usual seminomas merits investigation as a prognostic parameter.

AB - Despite its alarming appearance, spermatocytic seminoma virtually never metastasizes. We hypothesized that this paradox may at least be partially related to increased apoptosis compared to metastasizing germ cell tumors since high expression of proapoptotic factors correlates with indolent behavior in other tumor systems, notably CD30-positive cutaneous lymphoma, another neoplasm where phenotype and behavior do not match. We therefore compared apoptosis and apoptotic regulators in 17 spermatocytic seminomas (2 with sarcoma) and 18 usual seminomas by light microscopy and using immunostains for caspase-3, p53, bcl-2, bcl-xL, FADD, FAS and survivin. We found significantly greater numbers of apoptotic cells and activated caspase-3-positive cells in spermatocytic seminoma compared to usual seminoma (P<0.01). There was over a 10-fold range in apoptotic cells in usual seminoma but only a 4-fold variation in spermatocytic seminoma. Spermatocytic seminoma had decreased p53 expression compared to usual seminoma, with marked variation in bcl-2 expression and increased FADD. The two sarcomas in spermatocytic seminoma, however, showed decreased apoptosis and caspase-3 reactivity, with upregulation of p53 and bcl-2 and decreased FADD expression. We conclude that apoptosis, caspase-3 and FADD expression are increased in spermatocytic seminoma compared to usual seminoma. Apoptotic parameters are decreased in sarcomatous transformation of spermatocytic seminoma. The increased apoptosis of spermatocytic seminoma, possibly mediated by FAS independent activation of the death receptor pathway, may provide some insight into its excellent prognosis. The variation in apoptosis of usual seminomas merits investigation as a prognostic parameter.

KW - Apoptosis

KW - Proapoptotic factors

KW - Seminoma

KW - Spermatocytic seminoma

UR - http://www.scopus.com/inward/record.url?scp=34548765546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548765546&partnerID=8YFLogxK

U2 - 10.1038/modpathol.3800933

DO - 10.1038/modpathol.3800933

M3 - Article

C2 - 17643097

AN - SCOPUS:34548765546

VL - 20

SP - 1036

EP - 1044

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 10

ER -