Apoptosis in the endothelium of human corneas for transplantation

J. Albon, A. B. Tullo, S. Aktar, M. E. Boulton

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Purpose. To determine whether endothelial cell loss of human corneas stored in organ culture before transplantation is due to apoptosis. Methods. The corneal endothelium of human corneas, stored in organ culture at 34°C for varying periods of time, were analyzed for the presence of apoptotic cells using the TdT-mediated dUTP nick-end labeling (TUNEL) technique. Corneal endothelial cell apoptosis was confirmed by Hoechst staining and immunolabeling with anti-caspase 3 active antibody. Results. Apoptotic cells were identified in the corneal endothelium of human organ cultured corneas: their number and distribution demonstrated a close correlation with corneal folding and overall quality of the corneal endothelium. TUNEL-positive labeling of cells was confirmed as apoptotic by the presence of morphologic nuclear alterations identified by Hoechst staining and the presence of immunostaining for caspase-3 activity. Corneal endothelial cell apoptosis was independent of cause of donor death, death to enucleation time, and death to culture times. Conclusions. Corneal endothelial cell apoptosis appears to determine the suitability of a cornea for transplantation.

Original languageEnglish (US)
Pages (from-to)2887-2893
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number10
StatePublished - Sep 25 2000

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Corneal Transplantation
Corneal Endothelium
Endothelium
Endothelial Cells
Cornea
Apoptosis
Organ Culture Techniques
Caspase 3
Staining and Labeling
Cause of Death
Transplantation
Antibodies

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Albon, J., Tullo, A. B., Aktar, S., & Boulton, M. E. (2000). Apoptosis in the endothelium of human corneas for transplantation. Investigative Ophthalmology and Visual Science, 41(10), 2887-2893.

Apoptosis in the endothelium of human corneas for transplantation. / Albon, J.; Tullo, A. B.; Aktar, S.; Boulton, M. E.

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 10, 25.09.2000, p. 2887-2893.

Research output: Contribution to journalArticle

Albon, J, Tullo, AB, Aktar, S & Boulton, ME 2000, 'Apoptosis in the endothelium of human corneas for transplantation', Investigative Ophthalmology and Visual Science, vol. 41, no. 10, pp. 2887-2893.
Albon, J. ; Tullo, A. B. ; Aktar, S. ; Boulton, M. E. / Apoptosis in the endothelium of human corneas for transplantation. In: Investigative Ophthalmology and Visual Science. 2000 ; Vol. 41, No. 10. pp. 2887-2893.
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N2 - Purpose. To determine whether endothelial cell loss of human corneas stored in organ culture before transplantation is due to apoptosis. Methods. The corneal endothelium of human corneas, stored in organ culture at 34°C for varying periods of time, were analyzed for the presence of apoptotic cells using the TdT-mediated dUTP nick-end labeling (TUNEL) technique. Corneal endothelial cell apoptosis was confirmed by Hoechst staining and immunolabeling with anti-caspase 3 active antibody. Results. Apoptotic cells were identified in the corneal endothelium of human organ cultured corneas: their number and distribution demonstrated a close correlation with corneal folding and overall quality of the corneal endothelium. TUNEL-positive labeling of cells was confirmed as apoptotic by the presence of morphologic nuclear alterations identified by Hoechst staining and the presence of immunostaining for caspase-3 activity. Corneal endothelial cell apoptosis was independent of cause of donor death, death to enucleation time, and death to culture times. Conclusions. Corneal endothelial cell apoptosis appears to determine the suitability of a cornea for transplantation.

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