Apoptosis mediates cell death following traumatic injury in rat hippocampal neurons

P. T. Shah, K. W. Yoon, X. M. Xu, L. D. Broder

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A model of in vitro traumatic injury with dissociated rat hippocampal neurons was studied to explore the mechanism of cell death. The neurotoxicity induced by traumatic injury to the cell culture can be transferred to a naive uninjured culture by media exchange. This toxicity is attenuated by dimethyl- sulfoxide or superoxide dismutase, suggesting that this toxicity is mediated by a free radical generation. Ionotropic glutamate receptor antagonists had no effect. This toxicity was effectively blocked by the pretreatment of the naive uninjured recipient cultures with cycloheximide or with actinomycin D. The DNA fragmentation could be illustrated with in situ nick translation in the cells which seem to have lost their cytoplasm. The nuclear morphology of neurons labeled by a neurofilament-specific antibody, SMI-31, demonstrated chromatin condensation and nucleosome formation. Traumatic injury induces release of an unknown toxin into the extracellular space. These observations suggest that a traumatized neuronal culture can propagate cell death of naive uninjured cells by releasing a neurotoxin that causes apoptosis.

Original languageEnglish (US)
Pages (from-to)999-1004
Number of pages6
JournalNeuroscience
Volume79
Issue number4
DOIs
StatePublished - Jun 6 1997

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Cell Death
Apoptosis
Neurons
Wounds and Injuries
Ionotropic Glutamate Receptors
Excitatory Amino Acid Antagonists
Intermediate Filaments
Nucleosomes
Neurotoxins
Extracellular Space
Dactinomycin
DNA Fragmentation
Cycloheximide
Dimethyl Sulfoxide
Superoxide Dismutase
Chromatin
Free Radicals
Culture Media
Cytoplasm
Cell Culture Techniques

Keywords

  • Excitotoxicity
  • Free radicals
  • Ischemia
  • Programmed cell death

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Apoptosis mediates cell death following traumatic injury in rat hippocampal neurons. / Shah, P. T.; Yoon, K. W.; Xu, X. M.; Broder, L. D.

In: Neuroscience, Vol. 79, No. 4, 06.06.1997, p. 999-1004.

Research output: Contribution to journalArticle

Shah, P. T. ; Yoon, K. W. ; Xu, X. M. ; Broder, L. D. / Apoptosis mediates cell death following traumatic injury in rat hippocampal neurons. In: Neuroscience. 1997 ; Vol. 79, No. 4. pp. 999-1004.
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