Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal

Kirsten Lauber, Erwin Bohn, Stefan Martin Kröber, Yi Jin Xiao, Sibylle G. Blumenthal, Ralph K. Lindemann, Patrizia Marini, Carolin Wiedig, Anke Zobywalski, Shairaz Baksh, Yan Xu, Ingo B. Autenrieth, Klaus Schulze-Osthoff, Claus Belka, Gernot Stuhler, Sebastian Wesselborg

Research output: Contribution to journalArticle

591 Citations (Scopus)

Abstract

Efficient engulfment of the intact cell corpse is a critical end point of apoptosis, required to prevent secondary necrosis and inflammation. The presentation of "eat-me" signals on the dying cell is an important part of this process of recognition and engulfment by professional phagocytes. Here, we present evidence that apoptotic cells secrete chemotactic factor(s) that stimulate the attraction of monocytic cells and primary macrophages. The activation of caspase-3 in the apoptotic cell was found to be required for the release of this chemotactic factor(s). The putative chemoattractant was identified as the phospholipid, lysophosphatidylcholine. Further analysis showed that lysophosphatidylcholine was released from apoptotic cells due to the caspase-3 mediated activation of the calcium-independent phospholipase A2. These data suggest that in addition to eat-me signals, apoptotic cells display attraction signals to ensure the efficient removal of apoptotic cells and prevent postapoptotic necrosis.

Original languageEnglish (US)
Pages (from-to)717-730
Number of pages14
JournalCell
Volume113
Issue number6
DOIs
StatePublished - Jun 13 2003
Externally publishedYes

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Phagocytes
Caspase 3
Cell Movement
Cells
Chemotactic Factors
Lipids
Lysophosphatidylcholines
Calcium-Independent Phospholipase A2
Chemical activation
Macrophages
Necrosis
Phospholipids
Apoptosis
Cadaver
Inflammation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Lauber, K., Bohn, E., Kröber, S. M., Xiao, Y. J., Blumenthal, S. G., Lindemann, R. K., ... Wesselborg, S. (2003). Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal. Cell, 113(6), 717-730. https://doi.org/10.1016/S0092-8674(03)00422-7

Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal. / Lauber, Kirsten; Bohn, Erwin; Kröber, Stefan Martin; Xiao, Yi Jin; Blumenthal, Sibylle G.; Lindemann, Ralph K.; Marini, Patrizia; Wiedig, Carolin; Zobywalski, Anke; Baksh, Shairaz; Xu, Yan; Autenrieth, Ingo B.; Schulze-Osthoff, Klaus; Belka, Claus; Stuhler, Gernot; Wesselborg, Sebastian.

In: Cell, Vol. 113, No. 6, 13.06.2003, p. 717-730.

Research output: Contribution to journalArticle

Lauber, K, Bohn, E, Kröber, SM, Xiao, YJ, Blumenthal, SG, Lindemann, RK, Marini, P, Wiedig, C, Zobywalski, A, Baksh, S, Xu, Y, Autenrieth, IB, Schulze-Osthoff, K, Belka, C, Stuhler, G & Wesselborg, S 2003, 'Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal', Cell, vol. 113, no. 6, pp. 717-730. https://doi.org/10.1016/S0092-8674(03)00422-7
Lauber K, Bohn E, Kröber SM, Xiao YJ, Blumenthal SG, Lindemann RK et al. Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal. Cell. 2003 Jun 13;113(6):717-730. https://doi.org/10.1016/S0092-8674(03)00422-7
Lauber, Kirsten ; Bohn, Erwin ; Kröber, Stefan Martin ; Xiao, Yi Jin ; Blumenthal, Sibylle G. ; Lindemann, Ralph K. ; Marini, Patrizia ; Wiedig, Carolin ; Zobywalski, Anke ; Baksh, Shairaz ; Xu, Yan ; Autenrieth, Ingo B. ; Schulze-Osthoff, Klaus ; Belka, Claus ; Stuhler, Gernot ; Wesselborg, Sebastian. / Apoptotic cells induce migration of phagocytes via caspase-3-mediated release of a lipid attraction signal. In: Cell. 2003 ; Vol. 113, No. 6. pp. 717-730.
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