Apoptotic signaling induced by benzamide riboside: An in vitro study

Sujata Pathak, Chandresh Sharma, H. N. Jayaram, Neeta Singh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Benzamide riboside (BR) is a novel anticancer agent exhibiting potent cytotoxic activity in malignant cell lines. However, the mechanism of induction of apoptosis is not clear. The purpose of this study was to elucidate the apoptotic signaling induced by BR on different human cancer cell lines. Our results revealed that BR at a dose of 50 μM induces apoptosis in SiHa, Hep2, and Ca Ski cells as studied by morphology and flow cytometry. A downregulation of anti-apoptotic proteins Bcl-2 and Bcl-xL was observed, whereas the expression level of the pro-apoptotic protein Bax remained unaffected. An upregulation of p53 was observed while no change was seen on the level of apoptosis inducing factor (AIF). A significant increase in caspase-3 and -9 activities was seen, which was accompanied by PARP cleavage. Release of cytochrome c from the mitochondria to the cytosol was also observed. Taken together, the findings suggest that BR induces apoptosis in SiHa, Hep2, and Ca Ski cells via the intrinsic mitochondrial pathway.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume328
Issue number1-2
DOIs
StatePublished - 2009

Fingerprint

Apoptosis Regulatory Proteins
Apoptosis
Cells
Apoptosis Inducing Factor
Cell Line
Mitochondria
Flow cytometry
Caspase 9
Cytochromes c
Caspase 3
Antineoplastic Agents
Cytosol
Flow Cytometry
Up-Regulation
Down-Regulation
In Vitro Techniques
3-(1-deoxyribofuranosyl)benzamide
Neoplasms

Keywords

  • Apoptosis
  • Benzamide riboside
  • Caspase
  • Cytochrome c
  • IMPDH inhibitors
  • Mitochondria

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Apoptotic signaling induced by benzamide riboside : An in vitro study. / Pathak, Sujata; Sharma, Chandresh; Jayaram, H. N.; Singh, Neeta.

In: Molecular and Cellular Biochemistry, Vol. 328, No. 1-2, 2009, p. 67-73.

Research output: Contribution to journalArticle

Pathak, Sujata ; Sharma, Chandresh ; Jayaram, H. N. ; Singh, Neeta. / Apoptotic signaling induced by benzamide riboside : An in vitro study. In: Molecular and Cellular Biochemistry. 2009 ; Vol. 328, No. 1-2. pp. 67-73.
@article{cd92f4fe7cca44d2a804b95759a0d3bf,
title = "Apoptotic signaling induced by benzamide riboside: An in vitro study",
abstract = "Benzamide riboside (BR) is a novel anticancer agent exhibiting potent cytotoxic activity in malignant cell lines. However, the mechanism of induction of apoptosis is not clear. The purpose of this study was to elucidate the apoptotic signaling induced by BR on different human cancer cell lines. Our results revealed that BR at a dose of 50 μM induces apoptosis in SiHa, Hep2, and Ca Ski cells as studied by morphology and flow cytometry. A downregulation of anti-apoptotic proteins Bcl-2 and Bcl-xL was observed, whereas the expression level of the pro-apoptotic protein Bax remained unaffected. An upregulation of p53 was observed while no change was seen on the level of apoptosis inducing factor (AIF). A significant increase in caspase-3 and -9 activities was seen, which was accompanied by PARP cleavage. Release of cytochrome c from the mitochondria to the cytosol was also observed. Taken together, the findings suggest that BR induces apoptosis in SiHa, Hep2, and Ca Ski cells via the intrinsic mitochondrial pathway.",
keywords = "Apoptosis, Benzamide riboside, Caspase, Cytochrome c, IMPDH inhibitors, Mitochondria",
author = "Sujata Pathak and Chandresh Sharma and Jayaram, {H. N.} and Neeta Singh",
year = "2009",
doi = "10.1007/s11010-009-0075-8",
language = "English",
volume = "328",
pages = "67--73",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Apoptotic signaling induced by benzamide riboside

T2 - An in vitro study

AU - Pathak, Sujata

AU - Sharma, Chandresh

AU - Jayaram, H. N.

AU - Singh, Neeta

PY - 2009

Y1 - 2009

N2 - Benzamide riboside (BR) is a novel anticancer agent exhibiting potent cytotoxic activity in malignant cell lines. However, the mechanism of induction of apoptosis is not clear. The purpose of this study was to elucidate the apoptotic signaling induced by BR on different human cancer cell lines. Our results revealed that BR at a dose of 50 μM induces apoptosis in SiHa, Hep2, and Ca Ski cells as studied by morphology and flow cytometry. A downregulation of anti-apoptotic proteins Bcl-2 and Bcl-xL was observed, whereas the expression level of the pro-apoptotic protein Bax remained unaffected. An upregulation of p53 was observed while no change was seen on the level of apoptosis inducing factor (AIF). A significant increase in caspase-3 and -9 activities was seen, which was accompanied by PARP cleavage. Release of cytochrome c from the mitochondria to the cytosol was also observed. Taken together, the findings suggest that BR induces apoptosis in SiHa, Hep2, and Ca Ski cells via the intrinsic mitochondrial pathway.

AB - Benzamide riboside (BR) is a novel anticancer agent exhibiting potent cytotoxic activity in malignant cell lines. However, the mechanism of induction of apoptosis is not clear. The purpose of this study was to elucidate the apoptotic signaling induced by BR on different human cancer cell lines. Our results revealed that BR at a dose of 50 μM induces apoptosis in SiHa, Hep2, and Ca Ski cells as studied by morphology and flow cytometry. A downregulation of anti-apoptotic proteins Bcl-2 and Bcl-xL was observed, whereas the expression level of the pro-apoptotic protein Bax remained unaffected. An upregulation of p53 was observed while no change was seen on the level of apoptosis inducing factor (AIF). A significant increase in caspase-3 and -9 activities was seen, which was accompanied by PARP cleavage. Release of cytochrome c from the mitochondria to the cytosol was also observed. Taken together, the findings suggest that BR induces apoptosis in SiHa, Hep2, and Ca Ski cells via the intrinsic mitochondrial pathway.

KW - Apoptosis

KW - Benzamide riboside

KW - Caspase

KW - Cytochrome c

KW - IMPDH inhibitors

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=67650246578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650246578&partnerID=8YFLogxK

U2 - 10.1007/s11010-009-0075-8

DO - 10.1007/s11010-009-0075-8

M3 - Article

C2 - 19262994

AN - SCOPUS:67650246578

VL - 328

SP - 67

EP - 73

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -