Application of physicochemically modified silicon substrates as reverse-phase protein microarrays

A. Jasper Nijdam, Michael R. Zianni, Edward E. Herderick, Mark M C Cheng, Jenifer Prosperi, Fredika A. Robertson, Emanuel F. Petricoin, Lance A. Liotta, Mauro Ferrari

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Physicochemically modified silicon substrates can provide a high quality alternative to nitrocellulose-oated glass slides for use in reverse-phase protein microarrays. Enhancement of protein microarray sensitivities is an important goal, especially because molecular targets within patient tissues exist in low abundance. The ideal array substrate has a high protein binding affinity and low intrinsic background signal. Silicon, which has low intrinsic autofluorescence, is being explored as a potential microarray surface. In a previous paper (Nijdam, A. J.; Cheng, M. M.-C.; Fedele, R.; Geho, D. H.; Herrmann, P.; Killian, K.; Espina, V.; Petricoin, E. F.; Liotta, L. A.; Ferrari, M. Physicochemically Modified Silicon as Substrate for Protein Microarrays. Biomaterials 2007, 28, 550-558), it is shown that physicochemical modification of silicon substrates increases the binding of protein to silicon to a level comparable with that of nitrocellulose. Here, we apply such substrates in a reverse-phase protein microarray setting in two model systems.

Original languageEnglish (US)
Pages (from-to)1247-1254
Number of pages8
JournalJournal of Proteome Research
Volume8
Issue number3
DOIs
StatePublished - Mar 6 2009
Externally publishedYes

Fingerprint

Protein Array Analysis
Silicon
Microarrays
Substrates
Collodion
Proteins
Biocompatible Materials
Protein Binding
Glass
Carrier Proteins
Tissue

Keywords

  • Nanotechnology
  • Protein microarrays
  • Proteomics
  • Silicon

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Nijdam, A. J., Zianni, M. R., Herderick, E. E., Cheng, M. M. C., Prosperi, J., Robertson, F. A., ... Ferrari, M. (2009). Application of physicochemically modified silicon substrates as reverse-phase protein microarrays. Journal of Proteome Research, 8(3), 1247-1254. https://doi.org/10.1021/pr800455y

Application of physicochemically modified silicon substrates as reverse-phase protein microarrays. / Nijdam, A. Jasper; Zianni, Michael R.; Herderick, Edward E.; Cheng, Mark M C; Prosperi, Jenifer; Robertson, Fredika A.; Petricoin, Emanuel F.; Liotta, Lance A.; Ferrari, Mauro.

In: Journal of Proteome Research, Vol. 8, No. 3, 06.03.2009, p. 1247-1254.

Research output: Contribution to journalArticle

Nijdam, AJ, Zianni, MR, Herderick, EE, Cheng, MMC, Prosperi, J, Robertson, FA, Petricoin, EF, Liotta, LA & Ferrari, M 2009, 'Application of physicochemically modified silicon substrates as reverse-phase protein microarrays', Journal of Proteome Research, vol. 8, no. 3, pp. 1247-1254. https://doi.org/10.1021/pr800455y
Nijdam, A. Jasper ; Zianni, Michael R. ; Herderick, Edward E. ; Cheng, Mark M C ; Prosperi, Jenifer ; Robertson, Fredika A. ; Petricoin, Emanuel F. ; Liotta, Lance A. ; Ferrari, Mauro. / Application of physicochemically modified silicon substrates as reverse-phase protein microarrays. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 3. pp. 1247-1254.
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