Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential

Myka L. Estes, Julie A. Mund, Laura E. Mead, Daniel N. Prater, Shanbao Cai, Haiyan Wang, Karen E. Pollok, Michael P. Murphy, Caroline S.T. An, Edward F. Srour, David A. Ingram, Jamie Case

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Defining whether human circulating proangiogenic cells represent a subset of the hematopoietic system and express CD45 or are hematopoietic derivatives that do not express CD45 (and are called endothelial progenitor cells) remains controversial. We have previously developed a polychromatic flow cytometry (PFC) protocol to isolate subsets of hematopoietic cells and we now identify the circulating pool of CD34+CD45dim cells representing functional circulating hematopoietic stem and progenitor cells (CHSPCs) that can be separated on the basis of AC133 expression and report that the AC133 + subset of the CHSPCs enhances the growth of tumor blood vessels in vivo in immunodeficient mice. In addition, the ratio of AC1331 proangiogenic CHSPCs to AC133- nonangiogenic CHSPCs unambiguously correlates with the severity of the clinical state of patients with peripheral arterial disease. In sum, a PFC protocol validated via in vitro and in vivo analyses, can be used to interrogate the roles of human hematopoietic elements in the growth and maintenance of the vasculature.

Original languageEnglish (US)
Pages (from-to)831-839
Number of pages9
JournalCytometry Part A
Volume77
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • Angiogenesis
  • Circulating progenitor cells
  • Endothelial progenitor cells
  • Peripheral arterial disease
  • Polychromatic flow cytometry

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

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