Application of pressure cycling technology to tissue sample preparation for 2-DE

Heather Ringham, Richard L. Bell, Gary B. Smejkal, James Behnke, Frank A. Witzmann

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

2-DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post-translational modification. One of 2-DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology.

Original languageEnglish (US)
Pages (from-to)1022-1024
Number of pages3
JournalELECTROPHORESIS
Volume28
Issue number6
DOIs
StatePublished - Mar 1 2007

Keywords

  • 2-DE
  • Pressure cycling
  • Sample preparation
  • Solubilization

ASJC Scopus subject areas

  • Clinical Biochemistry

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