Application of pressure cycling technology to tissue sample preparation for 2-DE

Heather Ringham, Richard Bell, Gary B. Smejkal, James Behnke, Frank Witzmann

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

2-DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post-translational modification. One of 2-DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology.

Original languageEnglish
Pages (from-to)1022-1024
Number of pages3
JournalElectrophoresis
Volume28
Issue number6
DOIs
StatePublished - Mar 2007

Fingerprint

Tissue
Technology
Pressure
Proteins
Post Translational Protein Processing
Complex Mixtures
Gels

Keywords

  • 2-DE
  • Pressure cycling
  • Sample preparation
  • Solubilization

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Application of pressure cycling technology to tissue sample preparation for 2-DE. / Ringham, Heather; Bell, Richard; Smejkal, Gary B.; Behnke, James; Witzmann, Frank.

In: Electrophoresis, Vol. 28, No. 6, 03.2007, p. 1022-1024.

Research output: Contribution to journalArticle

Ringham, Heather ; Bell, Richard ; Smejkal, Gary B. ; Behnke, James ; Witzmann, Frank. / Application of pressure cycling technology to tissue sample preparation for 2-DE. In: Electrophoresis. 2007 ; Vol. 28, No. 6. pp. 1022-1024.
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