Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies

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28 Citations (Scopus)

Abstract

During immunostaining of specific proteins in tissue sections using monoclonal and polyclonal antibodies, visualization of general tissue staining/background or major structural features is helpful to pinpoint precise localization of the protein of interest. Often in skeletal muscle research, immunostaining with antibodies against connective tissue or plasma membrane proteins (collagen 1, laminin, and caveolin 3) are used for this purpose. Although immunostaining for these proteins works well, it is time consuming, costly, limits the number of antibodies against protein of interest that can be used on a single section, and is not applicable to some staining techniques. Lectins were frequently used in earlier publications for skeletal muscle fiber boundaries and connective tissue visualization, but are not common in the current research studies. This work investigates costaining of muscle, bone, ligament, and tendon tissue sections with fluorescently tagged wheat germ agglutinin (WGA) lectin as a tool for the visualization of connective tissue. The results of this study show that fluorescent WGA lectin costaining is a cost-effective, fast, and convenient method for connective tissue visualization, especially in the studies where extensive washes reduce staining of the structures that are the primary interest of the investigation.

Original languageEnglish (US)
Pages (from-to)18-22
Number of pages5
JournalMicroscopy Research and Technique
Volume74
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

connective tissue
tendons
skeletal muscle
ligaments
Wheat Germ Agglutinins
wheat
Ligaments
Tendons
staining
Connective Tissue
bones
Muscle
Bone
Skeletal Muscle
Visualization
Tissue
Staining and Labeling
proteins
Bone and Bones
antibodies

Keywords

  • Aggrecan
  • Immunostaining
  • Intramyocellular lipid droplets

ASJC Scopus subject areas

  • Anatomy
  • Instrumentation
  • Histology
  • Medical Laboratory Technology

Cite this

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abstract = "During immunostaining of specific proteins in tissue sections using monoclonal and polyclonal antibodies, visualization of general tissue staining/background or major structural features is helpful to pinpoint precise localization of the protein of interest. Often in skeletal muscle research, immunostaining with antibodies against connective tissue or plasma membrane proteins (collagen 1, laminin, and caveolin 3) are used for this purpose. Although immunostaining for these proteins works well, it is time consuming, costly, limits the number of antibodies against protein of interest that can be used on a single section, and is not applicable to some staining techniques. Lectins were frequently used in earlier publications for skeletal muscle fiber boundaries and connective tissue visualization, but are not common in the current research studies. This work investigates costaining of muscle, bone, ligament, and tendon tissue sections with fluorescently tagged wheat germ agglutinin (WGA) lectin as a tool for the visualization of connective tissue. The results of this study show that fluorescent WGA lectin costaining is a cost-effective, fast, and convenient method for connective tissue visualization, especially in the studies where extensive washes reduce staining of the structures that are the primary interest of the investigation.",
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KW - Aggrecan

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