Applications of ratio fluorescence microscopy in the study of cell physiology

Kenneth Dunn, Satyajit Mayor, Jeffrey N. Myers, Frederick R. Maxfield

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Quantitative fluorescence microscopy is becoming an increasingly important tool in the study of cell biology. Fluorescence microscopy has long been used for qualitative characterizations of subcellular distributions of proteins, lipids, nucleic acids, and ions, but quantifying these distributions is complicated by a variety of optical, biological, and physical factors. Many factors that complicate quantification of fluorescence in cells can be circumvented by analyzing fluorescence ratios derived from pairs of fluorescence images. In this review we will discuss the factors that affect fluorescence quantification, the advantages of quantifying fluorescence as a ratio, and give examples of how fluorescence ratio microscopy is being applied in studies of cell biology.

Original languageEnglish (US)
Pages (from-to)573-582
Number of pages10
JournalFASEB Journal
Volume8
Issue number9
StatePublished - 1994
Externally publishedYes

Fingerprint

cell physiology
Cell Physiological Phenomena
Fluorescence microscopy
Physiology
fluorescence microscopy
Fluorescence Microscopy
Fluorescence
fluorescence
Cytology
Cell Biology
cell biology
Biological Factors
Nucleic Acids
nucleic acids
microscopy
Ions
Microscopic examination
Lipids
ions
lipids

Keywords

  • calcium
  • endocytosis
  • membranes
  • pH
  • resonance energy transfer

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Dunn, K., Mayor, S., Myers, J. N., & Maxfield, F. R. (1994). Applications of ratio fluorescence microscopy in the study of cell physiology. FASEB Journal, 8(9), 573-582.

Applications of ratio fluorescence microscopy in the study of cell physiology. / Dunn, Kenneth; Mayor, Satyajit; Myers, Jeffrey N.; Maxfield, Frederick R.

In: FASEB Journal, Vol. 8, No. 9, 1994, p. 573-582.

Research output: Contribution to journalArticle

Dunn, K, Mayor, S, Myers, JN & Maxfield, FR 1994, 'Applications of ratio fluorescence microscopy in the study of cell physiology', FASEB Journal, vol. 8, no. 9, pp. 573-582.
Dunn, Kenneth ; Mayor, Satyajit ; Myers, Jeffrey N. ; Maxfield, Frederick R. / Applications of ratio fluorescence microscopy in the study of cell physiology. In: FASEB Journal. 1994 ; Vol. 8, No. 9. pp. 573-582.
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