Applications of tissue microdissection in cancer genetics.

Z. Zhuang, A. O. Vortmeyer

Research output: Contribution to journalReview article

28 Scopus citations

Abstract

A major obstacle to apply molecular biologic techniques effectively to the somatic genetic analysis of neoplastic tissue is the presence of abundant nonneoplastic elements in the analyzed specimen. These nonneoplastic elements, including reactive fibrous cells, vascular cells, and a variety of infiltrating white blood cells, may mask genetic alterations that otherwise would be easily detectable if the neoplastic cells were procured selectively. We have developed a microdissection technique which allows to selectively procure and genetically analyze small populations of neoplastic cells from the glass slide. In this report, we describe the microdissection technique and genetic analysis of archival frozen and paraffin-embedded tissue in general. Then, we review some implications of this approach of genetic analysis for mapping and cloning of new genes, identification of premalignant lesions, and differential analysis of different histologic components within the same tumor.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalCell vision : the journal of analytical morphology
Volume5
Issue number1
StatePublished - Jan 1 1998

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ASJC Scopus subject areas

  • Anatomy

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