Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) redox function negatively regulates NRF2

Melissa Fishel, Xue Wu, Cecilia M. Devlin, Derek P. Logsdon, Yanlin Jiang, Meihua Luo, Ying He, Zhangsheng Yu, Yan Tong, Kelsey P. Lipking, Anirban Maitra, N. V. Rajeshkumar, Glenda Scandura, Mark Kelley, Mircea Ivan

Research output: Contribution to journalArticle

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Abstract

Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) (henceforth referred to as Ref-1) is a multifunctional protein that in addition to its base excision DNA repair activity exerts redox control of multiple transcription factors, including nuclear factor κ-light chain enhancer of activated B cells (NF-κB), STAT3, activator protein-1 (AP-1), hypoxia-inducible factor-1 (HIF-1), and tumor protein 53 (p53). In recent years, Ref-1 has emerged as a promising therapeutic target in cancer, particularly in pancreatic ductal carcinoma. Although a significant amount of research has centered on Ref-1, no wide-ranging approach had been performed on the effects of Ref-1 inhibition and transcription factor activity perturbation. Starting with a broader approach, we identified a previously unsuspected effect on the nuclear factor erythroid-related factor 2 (NRF2), a critical regulator of cellular defenses against oxidative stress. Based on genetic and small molecule inhibitor-based methodologies, we demonstrated that repression of Ref-1 potently activates NRF2 and its downstream targets in a dose-dependent fashion, and that the redox, rather than the DNA repair function of Ref-1 is critical for this effect. Intriguingly, our results also indicate that this pathway does not involve reactive oxygen species. The link between Ref-1 and NRF2 appears to be present in all cells tested in vitro, noncancerous and cancerous, including patient-derived tumor samples. In particular, we focused on understanding the implications of the novel interaction between these two pathways in primary pancreatic ductal adenocarcinoma tumor cells and provide the first evidence that this mechanism has implications for overcoming the resistance against experimental drugs targeting Ref-1 activity, with clear translational implications.

Original languageEnglish
Pages (from-to)3057-3068
Number of pages12
JournalJournal of Biological Chemistry
Volume290
Issue number5
DOIs
StatePublished - Jan 30 2015

Fingerprint

DNA-(Apurinic or Apyrimidinic Site) Lyase
Endonucleases
Oxidation-Reduction
Tumors
DNA Repair
GATA1 Transcription Factor
Repair
Transcription Factors
Cells
Hypoxia-Inducible Factor 1
Neoplasms
Oxidative stress
Pancreatic Ductal Carcinoma
DNA
Transcription Factor AP-1
STAT3 Transcription Factor
Reactive Oxygen Species
Proteins
Drug Delivery Systems
Adenocarcinoma

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) redox function negatively regulates NRF2. / Fishel, Melissa; Wu, Xue; Devlin, Cecilia M.; Logsdon, Derek P.; Jiang, Yanlin; Luo, Meihua; He, Ying; Yu, Zhangsheng; Tong, Yan; Lipking, Kelsey P.; Maitra, Anirban; Rajeshkumar, N. V.; Scandura, Glenda; Kelley, Mark; Ivan, Mircea.

In: Journal of Biological Chemistry, Vol. 290, No. 5, 30.01.2015, p. 3057-3068.

Research output: Contribution to journalArticle

Fishel, M, Wu, X, Devlin, CM, Logsdon, DP, Jiang, Y, Luo, M, He, Y, Yu, Z, Tong, Y, Lipking, KP, Maitra, A, Rajeshkumar, NV, Scandura, G, Kelley, M & Ivan, M 2015, 'Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) redox function negatively regulates NRF2', Journal of Biological Chemistry, vol. 290, no. 5, pp. 3057-3068. https://doi.org/10.1074/jbc.M114.621995
Fishel, Melissa ; Wu, Xue ; Devlin, Cecilia M. ; Logsdon, Derek P. ; Jiang, Yanlin ; Luo, Meihua ; He, Ying ; Yu, Zhangsheng ; Tong, Yan ; Lipking, Kelsey P. ; Maitra, Anirban ; Rajeshkumar, N. V. ; Scandura, Glenda ; Kelley, Mark ; Ivan, Mircea. / Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) redox function negatively regulates NRF2. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 5. pp. 3057-3068.
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