Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells

Mitra R. de Cógáin, Michael P. Linnes, Hyo Jung Lee, Amy Krambeck, Julio Cezar de Mendonça Uchôa, Sung Hoon Kim, John C. Lieske

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Costus arabicus L. (C. arabicus) is a plant used in Brazilian folk medicine to treat urolithiasis; however, its mechanism of action is unclear. The interaction between calcium oxalate (CaOx) crystals and the renal epithelium is important in calculogenesis, and compounds that modulate this process represent candidate therapeutic agents for stone prevention. Therefore, we assessed the inhibitory activity of C. arabicus on CaOx crystallization and the interaction of CaOx crystals with the renal epithelium. A seeded CaOx monohydrate (COM) crystallization system was used to study the effect of C. arabicus on crystal growth. Madin Darby canine kidney (MDCK) cells were used to study [14C] COM crystal adhesion in the presence and absence of an aqueous extract of C. arabicus. Cytotoxicity was assessed using a tetrazolium (MTS) cell proliferation assay. Aqueous extracts of C. arabicus decreased crystal growth in a concentration-dependent fashion. Precoating crystals with C. arabicus extract prevented their adhesion to MDCK cells, while pretreating cells did not show any effect. The extract was non-cytotoxic in concentrations of at least 1 mg/ml, which is likely above concentrations achievable in the urine following oral ingestion and excretion. No inhibitory activity was found in hexane, methyl chloride, n-butanol and ethyl acetate fractions of an ethanol extract of the herb. An aqueous extract of C. arabicus may disrupt calculogenesis by interacting with CaOx crystal surfaces. Activity was present in the aqueous extract; therefore, this agent may be bioavailable when administered orally. Fractionation results suggest that the active agent might be a polar polysaccharide. Further identification and characterization along these lines may be warranted.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalUrolithiasis
Volume43
Issue number2
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Costus
Calcium Oxalate
Crystallization
Epithelial Cells
Kidney
Madin Darby Canine Kidney Cells
Epithelium
Methyl Chloride
1-Butanol
Urolithiasis
Hexanes
Traditional Medicine
Polysaccharides
Ethanol
Eating
Cell Proliferation
Urine

Keywords

  • Cell–crystal interaction
  • Costus arabicus
  • Crystallization
  • Herb
  • Nephrolithiasis
  • Traditional medicine

ASJC Scopus subject areas

  • Urology

Cite this

de Cógáin, M. R., Linnes, M. P., Lee, H. J., Krambeck, A., de Mendonça Uchôa, J. C., Kim, S. H., & Lieske, J. C. (2015). Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells. Urolithiasis, 43(2), 119-124. https://doi.org/10.1007/s00240-015-0749-5

Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells. / de Cógáin, Mitra R.; Linnes, Michael P.; Lee, Hyo Jung; Krambeck, Amy; de Mendonça Uchôa, Julio Cezar; Kim, Sung Hoon; Lieske, John C.

In: Urolithiasis, Vol. 43, No. 2, 2015, p. 119-124.

Research output: Contribution to journalArticle

de Cógáin, MR, Linnes, MP, Lee, HJ, Krambeck, A, de Mendonça Uchôa, JC, Kim, SH & Lieske, JC 2015, 'Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells', Urolithiasis, vol. 43, no. 2, pp. 119-124. https://doi.org/10.1007/s00240-015-0749-5
de Cógáin, Mitra R. ; Linnes, Michael P. ; Lee, Hyo Jung ; Krambeck, Amy ; de Mendonça Uchôa, Julio Cezar ; Kim, Sung Hoon ; Lieske, John C. / Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells. In: Urolithiasis. 2015 ; Vol. 43, No. 2. pp. 119-124.
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