Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection?

Troy A. Markel, Paul R. Crisostomo, Maiuxi C. Manukyan, Dalia Al-Azzawi, Christine M. Herring, Tim Lahm, Nathan M. Novotny, Daniel R. Meldrum

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Bone marrow stem cells (BMSCs) may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. However, stem cells from older hosts exhibit decreased function during stress. We therefore hypothesized that (1) BMSCs derived from neonatal hosts would provide protection to ischemic myocardium, and (2) neonatal stem cells would enhance postischemic myocardial recovery above that seen with adult stem cell therapy. Materials and methods: Female adult Sprague Dawley rat hearts were subjected to an ischemia/reperfusion protocol via Langendorff isolated heart preparation (15 min equilibration, 25 min ischemia, and 60 min reperfusion). BMSCs were harvested from adult and neonatal mice and cultured through several passages under normal conditions (37°C, 5% CO2/air). Immediately prior to ischemia, 1 million adult or neonatal BMSCs were infused into the coronary circulation. Cardiac functional parameters were continuously recorded. Results: Pretreatment with adult BMSCs significantly increased postischemic myocardial recovery as noted by improved left ventricular developed pressure, end diastolic pressure, contractility, and rate of relaxation. Neonatal stem cells, however, did not cause any noticeable improvement in myocardial functional parameters following ischemia. Conclusion: Neonatal and adult BMSCs are distinctly different in the degree of beneficial tissue protection that they can provide. The data herein suggests that a critical age exists as to when stem cells become fully activated to provide their beneficial protective properties. Defining the genes that initiate these protective properties may allow for genetic amplification of beneficial signals, and the generation of "super stem cells" that provide maximum protection to ischemic tissues.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalJournal of Surgical Research
Volume152
Issue number2
DOIs
StatePublished - Apr 2009

Fingerprint

Adult Stem Cells
Stem Cells
Bone Marrow Cells
Ischemia
Reperfusion
Coronary Circulation
Ventricular Pressure
Cell- and Tissue-Based Therapy
Sprague Dawley Rats
Myocardium
Air
Blood Pressure

Keywords

  • adult
  • estrogen
  • injury
  • myocardial
  • neonatal
  • VEGF

ASJC Scopus subject areas

  • Surgery

Cite this

Markel, T. A., Crisostomo, P. R., Manukyan, M. C., Al-Azzawi, D., Herring, C. M., Lahm, T., ... Meldrum, D. R. (2009). Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection? Journal of Surgical Research, 152(2), 325-330. https://doi.org/10.1016/j.jss.2008.03.054

Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection? / Markel, Troy A.; Crisostomo, Paul R.; Manukyan, Maiuxi C.; Al-Azzawi, Dalia; Herring, Christine M.; Lahm, Tim; Novotny, Nathan M.; Meldrum, Daniel R.

In: Journal of Surgical Research, Vol. 152, No. 2, 04.2009, p. 325-330.

Research output: Contribution to journalArticle

Markel, TA, Crisostomo, PR, Manukyan, MC, Al-Azzawi, D, Herring, CM, Lahm, T, Novotny, NM & Meldrum, DR 2009, 'Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection?', Journal of Surgical Research, vol. 152, no. 2, pp. 325-330. https://doi.org/10.1016/j.jss.2008.03.054
Markel, Troy A. ; Crisostomo, Paul R. ; Manukyan, Maiuxi C. ; Al-Azzawi, Dalia ; Herring, Christine M. ; Lahm, Tim ; Novotny, Nathan M. ; Meldrum, Daniel R. / Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection?. In: Journal of Surgical Research. 2009 ; Vol. 152, No. 2. pp. 325-330.
@article{7a49fd7d837b48e3ab0df0a16403bbc4,
title = "Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection?",
abstract = "Background: Bone marrow stem cells (BMSCs) may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. However, stem cells from older hosts exhibit decreased function during stress. We therefore hypothesized that (1) BMSCs derived from neonatal hosts would provide protection to ischemic myocardium, and (2) neonatal stem cells would enhance postischemic myocardial recovery above that seen with adult stem cell therapy. Materials and methods: Female adult Sprague Dawley rat hearts were subjected to an ischemia/reperfusion protocol via Langendorff isolated heart preparation (15 min equilibration, 25 min ischemia, and 60 min reperfusion). BMSCs were harvested from adult and neonatal mice and cultured through several passages under normal conditions (37°C, 5{\%} CO2/air). Immediately prior to ischemia, 1 million adult or neonatal BMSCs were infused into the coronary circulation. Cardiac functional parameters were continuously recorded. Results: Pretreatment with adult BMSCs significantly increased postischemic myocardial recovery as noted by improved left ventricular developed pressure, end diastolic pressure, contractility, and rate of relaxation. Neonatal stem cells, however, did not cause any noticeable improvement in myocardial functional parameters following ischemia. Conclusion: Neonatal and adult BMSCs are distinctly different in the degree of beneficial tissue protection that they can provide. The data herein suggests that a critical age exists as to when stem cells become fully activated to provide their beneficial protective properties. Defining the genes that initiate these protective properties may allow for genetic amplification of beneficial signals, and the generation of {"}super stem cells{"} that provide maximum protection to ischemic tissues.",
keywords = "adult, estrogen, injury, myocardial, neonatal, VEGF",
author = "Markel, {Troy A.} and Crisostomo, {Paul R.} and Manukyan, {Maiuxi C.} and Dalia Al-Azzawi and Herring, {Christine M.} and Tim Lahm and Novotny, {Nathan M.} and Meldrum, {Daniel R.}",
year = "2009",
month = "4",
doi = "10.1016/j.jss.2008.03.054",
language = "English",
volume = "152",
pages = "325--330",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Are Neonatal Stem Cells as Effective as Adult Stem Cells in Providing Ischemic Protection?

AU - Markel, Troy A.

AU - Crisostomo, Paul R.

AU - Manukyan, Maiuxi C.

AU - Al-Azzawi, Dalia

AU - Herring, Christine M.

AU - Lahm, Tim

AU - Novotny, Nathan M.

AU - Meldrum, Daniel R.

PY - 2009/4

Y1 - 2009/4

N2 - Background: Bone marrow stem cells (BMSCs) may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. However, stem cells from older hosts exhibit decreased function during stress. We therefore hypothesized that (1) BMSCs derived from neonatal hosts would provide protection to ischemic myocardium, and (2) neonatal stem cells would enhance postischemic myocardial recovery above that seen with adult stem cell therapy. Materials and methods: Female adult Sprague Dawley rat hearts were subjected to an ischemia/reperfusion protocol via Langendorff isolated heart preparation (15 min equilibration, 25 min ischemia, and 60 min reperfusion). BMSCs were harvested from adult and neonatal mice and cultured through several passages under normal conditions (37°C, 5% CO2/air). Immediately prior to ischemia, 1 million adult or neonatal BMSCs were infused into the coronary circulation. Cardiac functional parameters were continuously recorded. Results: Pretreatment with adult BMSCs significantly increased postischemic myocardial recovery as noted by improved left ventricular developed pressure, end diastolic pressure, contractility, and rate of relaxation. Neonatal stem cells, however, did not cause any noticeable improvement in myocardial functional parameters following ischemia. Conclusion: Neonatal and adult BMSCs are distinctly different in the degree of beneficial tissue protection that they can provide. The data herein suggests that a critical age exists as to when stem cells become fully activated to provide their beneficial protective properties. Defining the genes that initiate these protective properties may allow for genetic amplification of beneficial signals, and the generation of "super stem cells" that provide maximum protection to ischemic tissues.

AB - Background: Bone marrow stem cells (BMSCs) may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. However, stem cells from older hosts exhibit decreased function during stress. We therefore hypothesized that (1) BMSCs derived from neonatal hosts would provide protection to ischemic myocardium, and (2) neonatal stem cells would enhance postischemic myocardial recovery above that seen with adult stem cell therapy. Materials and methods: Female adult Sprague Dawley rat hearts were subjected to an ischemia/reperfusion protocol via Langendorff isolated heart preparation (15 min equilibration, 25 min ischemia, and 60 min reperfusion). BMSCs were harvested from adult and neonatal mice and cultured through several passages under normal conditions (37°C, 5% CO2/air). Immediately prior to ischemia, 1 million adult or neonatal BMSCs were infused into the coronary circulation. Cardiac functional parameters were continuously recorded. Results: Pretreatment with adult BMSCs significantly increased postischemic myocardial recovery as noted by improved left ventricular developed pressure, end diastolic pressure, contractility, and rate of relaxation. Neonatal stem cells, however, did not cause any noticeable improvement in myocardial functional parameters following ischemia. Conclusion: Neonatal and adult BMSCs are distinctly different in the degree of beneficial tissue protection that they can provide. The data herein suggests that a critical age exists as to when stem cells become fully activated to provide their beneficial protective properties. Defining the genes that initiate these protective properties may allow for genetic amplification of beneficial signals, and the generation of "super stem cells" that provide maximum protection to ischemic tissues.

KW - adult

KW - estrogen

KW - injury

KW - myocardial

KW - neonatal

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=61849153442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849153442&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2008.03.054

DO - 10.1016/j.jss.2008.03.054

M3 - Article

VL - 152

SP - 325

EP - 330

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -