ARID3B increases ovarian tumor burden and is associated with a cancer stem cell gene signature

Lynn Roy, Serene J. Samyesudhas, Martin Carrasco, Jun Li, Stancy Joseph, Richard Dahl, Karen D. Cowden Dahl

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Ovarian cancer is the most deadly gynecological malignancy since most patients have metastatic disease at the time of diagnosis. Therefore, identification of critical pathways that contribute to ovarian cancer progression is necessary to yield novel therapeutic targets. Recently we reported that the DNA binding protein ARID3B is overexpressed in human ovarian tumors. To determine if ARID3B has oncogenic functions in vivo, ovarian cancer cell lines stably expressing ARID3B were injected intraperitoneally into nude mice. Overexpression of ARID3B increased tumor burden and decreased survival. To assess how ARID3B contributes to the increased tumor growth in vivo, we identified ARID3B induced genes in tumor ascites cells. ARID3B induced expression of genes associated with metastasis and cancer stem cells (CD44, LGR5, PROM1 (CD133), and Notch2). Moreover, ARID3B increased the number of CD133+ (a cancer stem cell marker) cells compared to control cells. The increase in CD133+ cells resulting from ARID3B expression was accompanied by enhanced paclitaxel resistance. Our data demonstrate that ARID3B boosts production of CD133+ cells and increases ovarian cancer progression in vivo.

Original languageEnglish (US)
Pages (from-to)8355-8366
Number of pages12
JournalOncotarget
Volume5
Issue number18
DOIs
StatePublished - 2014

Keywords

  • Cancer stem cells
  • Metastasis
  • Ovarian cancer
  • Transcription factor
  • Xenografts

ASJC Scopus subject areas

  • Oncology
  • Medicine(all)

Fingerprint Dive into the research topics of 'ARID3B increases ovarian tumor burden and is associated with a cancer stem cell gene signature'. Together they form a unique fingerprint.

  • Cite this

    Roy, L., Samyesudhas, S. J., Carrasco, M., Li, J., Joseph, S., Dahl, R., & Cowden Dahl, K. D. (2014). ARID3B increases ovarian tumor burden and is associated with a cancer stem cell gene signature. Oncotarget, 5(18), 8355-8366. https://doi.org/10.18632/oncotarget.2247