Asp129 of low molecular weight protein tyrosine phosphatase is involved in leaving group protonation

Zhong-Yin Zhang, E. Harms, R. L. Van Etten

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Site-directed mutagenesis was used to explore the functions of a number of acidic residues of bovine low molecular weight protein tyrosine phosphatase. Residues Asp-129, Asp-56, and Asp-92 were mutated to Ala or Asn. The mutant enzymes D56A, D56N, and D92A showed no significant changes in V(max) values, although they did exhibit significantly altered K(m) values. In contrast, the D129A mutant enzyme exhibited a greater than 2000-fold reduction in V(max), using p-nitrophenyl phosphate as a substrate. The V(max) values of D129A also exhibited a leaving group dependence, an altered solvent isotope effect of V(max)/(H)/V(max)/(D) of 0.78, and a lack of dependence on the presence of alternative phosphate acceptor alcohols, all properties that distinguish this mutant from wild type enzyme. The differences are due to a change of the rate-limiting step of the catalytic reaction. Asp-129 is concluded to be the proton donor to the leaving group in the phosphorylation step, and its mutation to alanine results in a reduced V(max) value and a change in the rate-limiting step of the catalysis from dephosphorylation to phosphorylation. Mechanistic considerations suggest that other phosphotyrosyl phosphatases having cysteine at the active site may be expected to have a similar requirement for a proton donor.

Original languageEnglish (US)
Pages (from-to)25947-25950
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number42
StatePublished - Jan 1 1994
Externally publishedYes

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Protein Tyrosine Phosphatases
Protonation
Phosphorylation
Molecular Weight
Molecular weight
Protons
Enzymes
Mutagenesis
Site-Directed Mutagenesis
Catalysis
Phosphoric Monoester Hydrolases
Isotopes
Alanine
Cysteine
Catalytic Domain
Phosphates
Alcohols
Mutation
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Asp129 of low molecular weight protein tyrosine phosphatase is involved in leaving group protonation. / Zhang, Zhong-Yin; Harms, E.; Van Etten, R. L.

In: Journal of Biological Chemistry, Vol. 269, No. 42, 01.01.1994, p. 25947-25950.

Research output: Contribution to journalArticle

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