Assay of plasma testosterone during the first six months of life: Importance of chromatographic purification of steroids

John S. Fuqua, Ellen S. Sher, Claude J. Migeon, Gary D. Berkovitz

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Determination of the plasma concentration of testosterone (T) is important in evaluating infants born with ambiguous genitalia and micropenis, and several commercially available kits provide a direct assay of T in unextracted plasma. Using plasma samples obtained from 36 subjects <6 months old, we compared the concentration of plasma T measured by RIA after extraction and purification by column chromatography with the T concentration measured in a direct assay. When aliquots of samples were purified before RIA, the concentration of T was markedly lower than in the direct assay. In the first 3 weeks postpartum, results of the direct assay were 3.8-fold greater than those obtained after purification. This difference decreased over time, and by age 2 months there was fairly good agreement between the two methods. These data indicate that some direct assays of plasma T are inappropriate during the first 2 months postpartum.

Original languageEnglish (US)
Pages (from-to)1146-1149
Number of pages4
JournalClinical chemistry
Volume41
Issue number8
DOIs
StatePublished - 1995

Keywords

  • cryptorchidism
  • intermethod comparison
  • neonates
  • radioimmunoassay
  • sample preparation
  • sex differentiation disorders

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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