Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase

Nam Ho Jeoung, Paresh C. Sanghani, Lanmin Zhai, Robert Harris

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The activity of the pyruvate dehydrogenase complex has long been determined in some laboratories by coupling the production of acetyl-coenzyme A (acetyl-CoA) to the acetylation of 4-aminoazobenzene-4′-sulfonic acid by arylamine N-acetyltransferase. The assay has some advantages, but its use has been limited by the need for large amounts of arylamine N-acetyltransferase. Here we report production of recombinant chicken liver arylamine N-acetyltransferase and optimization of its use in miniaturized assays for the pyruvate dehydrogenase complex and its kinase.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalAnalytical Biochemistry
Volume356
Issue number1
DOIs
StatePublished - Sep 1 2006

Fingerprint

Arylamine N-Acetyltransferase
Pyruvate Dehydrogenase Complex
Liver
Chickens
Assays
Acetylation
Acetyl Coenzyme A
Sulfonic Acids
Phosphotransferases

Keywords

  • 4-Aminoazobenzene-4′-sulfonic acid
  • Acetyl-CoA
  • Arylamine N-acetyltransferase
  • Pyruvate dehydrogenase complex
  • Pyruvate dehydrogenase kinase
  • Pyruvate dehydrogenase phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase. / Jeoung, Nam Ho; Sanghani, Paresh C.; Zhai, Lanmin; Harris, Robert.

In: Analytical Biochemistry, Vol. 356, No. 1, 01.09.2006, p. 44-50.

Research output: Contribution to journalArticle

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