Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase

Nam Ho Jeoung, Paresh C. Sanghani, Lanmin Zhai, Robert A. Harris

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The activity of the pyruvate dehydrogenase complex has long been determined in some laboratories by coupling the production of acetyl-coenzyme A (acetyl-CoA) to the acetylation of 4-aminoazobenzene-4′-sulfonic acid by arylamine N-acetyltransferase. The assay has some advantages, but its use has been limited by the need for large amounts of arylamine N-acetyltransferase. Here we report production of recombinant chicken liver arylamine N-acetyltransferase and optimization of its use in miniaturized assays for the pyruvate dehydrogenase complex and its kinase.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalAnalytical biochemistry
Volume356
Issue number1
DOIs
StatePublished - Sep 1 2006

Keywords

  • 4-Aminoazobenzene-4′-sulfonic acid
  • Acetyl-CoA
  • Arylamine N-acetyltransferase
  • Pyruvate dehydrogenase complex
  • Pyruvate dehydrogenase kinase
  • Pyruvate dehydrogenase phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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