Assessing new bone formation in neonatal calvarial organ cultures

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Citations (Scopus)

Abstract

Bone formation is a complex process, and testing anabolic effects on the skeleton of agents is slow and expensive in animals. Neonatal mouse calvariae cultured ex vivo show strong anabolic or catabolic bone responses to 1-week treatments and can be analyzed by quantitative histomorphometry. Changes in new bone area and osteoblast number caused by added proteins, drugs, or transfected genes can be quantified and analyzed for statistical significance. The organ cultures preserve much of the skeletal architecture and cellular diversity present in vivo and offer greater physiological relevance than cell lines studied in vitro.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages37-50
Number of pages14
Volume455
ISBN (Print)9781588298287
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume455
ISSN (Print)10643745

Fingerprint

Organ Culture Techniques
Osteogenesis
Anabolic Agents
Bone and Bones
Osteoblasts
Skull
Skeleton
Cell Line
Pharmaceutical Preparations
Genes
Proteins
In Vitro Techniques

Keywords

  • Bone formation
  • Neonatal calvaria
  • Organ culture
  • Osteoblast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mohammad, K., Chirgwin, J., & Guise, T. (2008). Assessing new bone formation in neonatal calvarial organ cultures. In Methods in Molecular Biology (Vol. 455, pp. 37-50). (Methods in Molecular Biology; Vol. 455). Humana Press. https://doi.org/10.1007/978-1-59745-104-8_3

Assessing new bone formation in neonatal calvarial organ cultures. / Mohammad, Khalid; Chirgwin, John; Guise, Theresa.

Methods in Molecular Biology. Vol. 455 Humana Press, 2008. p. 37-50 (Methods in Molecular Biology; Vol. 455).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mohammad, K, Chirgwin, J & Guise, T 2008, Assessing new bone formation in neonatal calvarial organ cultures. in Methods in Molecular Biology. vol. 455, Methods in Molecular Biology, vol. 455, Humana Press, pp. 37-50. https://doi.org/10.1007/978-1-59745-104-8_3
Mohammad K, Chirgwin J, Guise T. Assessing new bone formation in neonatal calvarial organ cultures. In Methods in Molecular Biology. Vol. 455. Humana Press. 2008. p. 37-50. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-104-8_3
Mohammad, Khalid ; Chirgwin, John ; Guise, Theresa. / Assessing new bone formation in neonatal calvarial organ cultures. Methods in Molecular Biology. Vol. 455 Humana Press, 2008. pp. 37-50 (Methods in Molecular Biology).
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