Assessing new bone formation in neonatal calvarial organ cultures

Research output: Chapter in Book/Report/Conference proceedingChapter

27 Scopus citations


Bone formation is a complex process, and testing anabolic effects on the skeleton of agents is slow and expensive in animals. Neonatal mouse calvariae cultured ex vivo show strong anabolic or catabolic bone responses to 1-week treatments and can be analyzed by quantitative histomorphometry. Changes in new bone area and osteoblast number caused by added proteins, drugs, or transfected genes can be quantified and analyzed for statistical significance. The organ cultures preserve much of the skeletal architecture and cellular diversity present in vivo and offer greater physiological relevance than cell lines studied in vitro.

Original languageEnglish (US)
Title of host publicationOsteoporosis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Number of pages14
ISBN (Print)9781588298287
StatePublished - Jan 1 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bone formation
  • Neonatal calvaria
  • Organ culture
  • Osteoblast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Mohammad, K. S., Chirgwin, J. M., & Guise, T. A. (2008). Assessing new bone formation in neonatal calvarial organ cultures. In Osteoporosis: Methods and Protocols (pp. 37-50). (Methods in Molecular Biology; Vol. 455). Humana Press.