Assessment of cardiac conduction: Basic principles of optical mapping

Chunhua Ding, Thomas H. Everett IV

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Extracellular recordings acquired from electrodes placed on the surface of cardiac tissue have traditionally been used to study the electrophysiological properties of the tissue. While this technique has been used in several studies that have increased our understanding of cardiac arrhythmias and action potential propagation, there are several limitations that have prevented us from seeing a bigger picture of arrhythmia mechanisms. These limitations include the limited number of electrodes and unstable recordings. Optical mapping was developed to increase the temporal and spatial resolution over traditional electrode recordings and ultimately the accuracy of the data analysis. This technology involves using a voltage-sensitive dye that binds to the cell membrane. The fluorescence changes of the dye have a linear relationship to the action potential changes of the cell membrane. These fluorescent changes can then be detected by a photodiode array, a CCD camera or a CMOS camera. This will allow the recording of the action potential in hundreds to thousands of different sites simultaneously. Presented in this chapter are the materials and hardware needed along with step-by-step instructions on setup and techniques used in optical mapping for larger tissue preparations.

Original languageEnglish (US)
Title of host publicationStem Cells for Myocardial Regeneration
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages239-252
Number of pages14
ISBN (Print)9781607617044
DOIs
StatePublished - Jan 1 2010

Publication series

NameMethods in Molecular Biology
Volume660
ISSN (Print)1064-3745

Fingerprint

Action Potentials
Electrodes
Cardiac Arrhythmias
Coloring Agents
Cell Membrane
Fluorescence
Technology
Data Accuracy

Keywords

  • Conduction
  • Electrophysiology
  • Optical mapping

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ding, C., & Everett IV, T. H. (2010). Assessment of cardiac conduction: Basic principles of optical mapping. In Stem Cells for Myocardial Regeneration: Methods and Protocols (pp. 239-252). (Methods in Molecular Biology; Vol. 660). Humana Press Inc.. https://doi.org/10.1007/978-1-60761-705-1_15

Assessment of cardiac conduction : Basic principles of optical mapping. / Ding, Chunhua; Everett IV, Thomas H.

Stem Cells for Myocardial Regeneration: Methods and Protocols. Humana Press Inc., 2010. p. 239-252 (Methods in Molecular Biology; Vol. 660).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ding, C & Everett IV, TH 2010, Assessment of cardiac conduction: Basic principles of optical mapping. in Stem Cells for Myocardial Regeneration: Methods and Protocols. Methods in Molecular Biology, vol. 660, Humana Press Inc., pp. 239-252. https://doi.org/10.1007/978-1-60761-705-1_15
Ding C, Everett IV TH. Assessment of cardiac conduction: Basic principles of optical mapping. In Stem Cells for Myocardial Regeneration: Methods and Protocols. Humana Press Inc. 2010. p. 239-252. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-705-1_15
Ding, Chunhua ; Everett IV, Thomas H. / Assessment of cardiac conduction : Basic principles of optical mapping. Stem Cells for Myocardial Regeneration: Methods and Protocols. Humana Press Inc., 2010. pp. 239-252 (Methods in Molecular Biology).
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