Assessment of corneal wound repair in vitro

Stephen Tuft, Peter Eggli, Mike Boulton, John Marshall

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The time course for the closure of linear wounds produced in cultured cell monolayers following either mechanical debridement or excimer laser photoablation were compared. Wounds were produced in the central zone of a confluent layer of bovine corneal epithelial cells, keratocytes, or endothelial cells, and the residual area of the wound measured at regular intervals. Differences in the rate of wound closure between cell types were demonstrated. For epithelial cells the rate of resurfacing after mechanical or excimer laser damage was similar; for keratocytes there was a lag period before cell slide began after laser injury, but the subsequent rate of resurfacing was then similar to mechanical wounds; only for endothelial layers was a significantly reduced rate of closure observed after photoablation as compared to mechanical wounding. In this model of wound healing it appears that it is the mechanism of removal of the extracellular matrix formed by endothelial cells that produces the major difference in the rate of wound closure between mechanical injury or photoablation.

Original languageEnglish (US)
Pages (from-to)713-719
Number of pages7
JournalCurrent Eye Research
Volume8
Issue number7
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Wounds and Injuries
Excimer Lasers
Endothelial Cells
Epithelial Cells
In Vitro Techniques
Debridement
Wound Healing
Extracellular Matrix
Cultured Cells
Lasers

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Tuft, S., Eggli, P., Boulton, M., & Marshall, J. (1989). Assessment of corneal wound repair in vitro. Current Eye Research, 8(7), 713-719. https://doi.org/10.3109/02713688909025806

Assessment of corneal wound repair in vitro. / Tuft, Stephen; Eggli, Peter; Boulton, Mike; Marshall, John.

In: Current Eye Research, Vol. 8, No. 7, 1989, p. 713-719.

Research output: Contribution to journalArticle

Tuft, S, Eggli, P, Boulton, M & Marshall, J 1989, 'Assessment of corneal wound repair in vitro', Current Eye Research, vol. 8, no. 7, pp. 713-719. https://doi.org/10.3109/02713688909025806
Tuft, Stephen ; Eggli, Peter ; Boulton, Mike ; Marshall, John. / Assessment of corneal wound repair in vitro. In: Current Eye Research. 1989 ; Vol. 8, No. 7. pp. 713-719.
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