Assessment of Cu-ETS as a PET radiopharmaceutical for evaluation of regional renal perfusion

Mark A. Green, Carla J. Mathias, Lynn R. Willis, Rajash K. Handa, Jeffrey L. Lacy, Michael A. Miller, Gary D. Hutchins

Research output: Contribution to journalArticle

42 Scopus citations


The copper(II) complex of ethylglyoxal bis(thiosemicarbazone) (Cu-ETS) was evaluated as a positron emission tomography (PET) radiopharmaceutical for assessment of regional renal perfusion. Methods: The concordance of renal flow estimates obtained with 11- and 15-μm microspheres was confirmed in four immature farm pigs using co-injected 46Sc- and 57Co-microspheres administered into the left ventricle. With the use of both immature farm pigs (n=3) and mature Göttingen minipigs (n=6), regional renal radiocopper uptake following intravenous [64Cu]Cu-ETS administration was compared to microsphere measurements of renal perfusion. The distribution and kinetics of [64Cu]Cu-ETS were further studied by PET imaging of the kidneys. The rate of [64Cu]Cu-ETS decomposition by blood was evaluated in vitro, employing octanol extraction to recover intact [64Cu]Cu-ETS. Results: The co-injected 11- and 15-μm microspheres provided similar estimates of renal flow. A linear relationship was observed between the renal uptake of intravenous [64Cu]Cu-ETS and regional renal perfusion measured using microspheres. [64Cu]Cu-ETS provided high-quality PET kidney images demonstrating the expected count gradient from high-flow outer cortex to low-flow medulla. When incubated with pig blood in vitro at 37°C, the [64Cu]Cu-ETS radiopharmaceutical was observed to decompose with a half-time of 2.8 min. Conclusion: Cu-ETS appears suitable for use as a PET radiopharmaceutical for evaluation of regional renal perfusion, affording renal uptake of radiocopper that varies linearly with microsphere perfusion measurements. Quantification of renal perfusion (in ml min-1 g-1) with [60,61,62,64Cu]Cu-ETS will require correcting the arterial input function for the fraction of blood radiocopper remaining present as the intact Cu-ETS radiopharmaceutical, since the Cu-ETS chelate has limited chemical stability in blood. Rapid octanol extraction of blood samples appears suitable as an approach to capturing the actual blood concentration of [60/61/62/64Cu]Cu-ETS.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalNuclear Medicine and Biology
Issue number3
StatePublished - Apr 1 2007


  • Copper-62
  • Copper-64
  • Cu-ETS radiopharmaceutical
  • Positron emission tomography (PET)
  • Renal perfusion

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

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