Assessment of PCNA (19A2) and Ki-67 (MIB1) cell proliferation markers in formalin fixed tissues

K. P. Teter, D. C. Holloway, George Sandusky

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Studies have shown that PCNA and Ki-67 increase in late G1 phase, become maximal in S phase and decline in G2 and M phase of the cell cycle. Correlation of increased PCNA and Ki-67 immunostaining with tissue injury, repair, and tumor cell growth has been observed. In the present study, the immunohistochemical staining pattern of formalin fixed ileum from man, dog, cat, pig, rabbit, rat and mouse were examined. Liver was also examined in dog, cat, pig, rat, and mouse. PCNA staining was superior to Ki-67 staining in all species examined except the pig and rabbit. Ki-67 staining was stronger than PCNA only in the rabbit. In the rat and mouse, the Ki-67 antibody stained goblet cells in the GI tract and not the cell nuclei, as should have been observed. Overall, the PCNA antibody was better than the Ki- 67 antibody for determining cell proliferation in the various animal species examined.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalJournal of Histotechnology
Volume18
Issue number2
StatePublished - 1995
Externally publishedYes

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Proliferating Cell Nuclear Antigen
Formaldehyde
Cell Proliferation
Staining and Labeling
Swine
Rabbits
Antibodies
Cats
Dogs
Goblet Cells
G2 Phase
G1 Phase
Cell Nucleus
S Phase
Ileum
Cell Division
Gastrointestinal Tract
Cell Cycle
Liver
Wounds and Injuries

Keywords

  • cell proliferation markers
  • Ki-67
  • PCNA

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Assessment of PCNA (19A2) and Ki-67 (MIB1) cell proliferation markers in formalin fixed tissues. / Teter, K. P.; Holloway, D. C.; Sandusky, George.

In: Journal of Histotechnology, Vol. 18, No. 2, 1995, p. 99-104.

Research output: Contribution to journalArticle

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