Studies have shown that PCNA and Ki-67 increase in lateG1 phase, become maximal in S phase and decline in G2 and M phase of the cell cycle. Correlation of increased PCNA and Ki- 67 immunostaining with tissue injury, repair, and tumor cell growth has been observed. In the present study, the immunohistochemical staining pattern of formalin fixed ileum from man, dog, cat, pig, rabbit, rat and mouse were examined. Liver was also examined in dog, cat, pig, rat, and mouse. PCNA staining was superior to Ki-67 staining in all species examined except the pig and rabbit. Ki-67 staining was stronger than PCNA only in the rabbit. In the rat and mouse, the Ki-67 antibody stained goblet cells in the GI tract and not the cell nuclei, as should have been observed. Overall, the PCNA antibody was better than the Ki-67 antibody for determining cell proliferation in the various animal species examined. (The J Histotechnol 18:99, 1995)w.
- Cell proliferation markers
ASJC Scopus subject areas
- Medical Laboratory Technology