Association of HPV types 6, 11, 16, and 18 DNA detection and serological response in unvaccinated adolescent women

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7 Citations (Scopus)

Abstract

Antibodies directed against the human papillomavirus (HPV) L1 protein are detected in approximately 70% of individuals with HPV infections. The factors associated with a serological response are not characterized. It is hypothesized that the HPV viral load, duration of detection, or both would be associated with seropositivity in adolescent women. Adolescent women (n=117), ages 15-17 at enrolment were followed for a mean of 6.2 years. Quarterly vaginal swabs (mean 22 per participant) were used to identify HPV 6, 11, 16, or 18 DNA (Roche PCR/Linear Array). Type-specific HPV infection was defined as ≥2 positive assays. To approximate viral load, Roche PCR/Linear Array test strips were scored visually based on the strength of signal relative to beta-globin controls. Sera collected near the end of study were tested by cLIA. Regression models were fit to assess associations between strength of signal (as represented by mean and cumulative strength of signal), duration of HPV detection, seropositivity, and serotiter. Detection of HPV DNA was associated with seropositivity for four types combined and for types 6, 16, and 18. Overall, 70.1% of DNA positive episodes were associated with type-specific seropositivity. The cumulative HPV DNA signal strength during periods of HPV detection for types 6, 11, 16, and 18 combined was associated with seropositivity (OR=1.21, 95% CI 1.02-1.44 P=0.026). No other HPV DNA predictors were found to be associated with seropositivity or serotiter.

Original languageEnglish
Pages (from-to)1786-1793
Number of pages8
JournalJournal of Medical Virology
Volume85
Issue number10
DOIs
StatePublished - Oct 2013

Fingerprint

Human papillomavirus 11
Human papillomavirus 6
DNA
Papillomavirus Infections
Viral Load
Polymerase Chain Reaction
beta-Globins
Antibodies
Serum

Keywords

  • Adolescent women
  • Antibody response
  • Human papillomavirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

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title = "Association of HPV types 6, 11, 16, and 18 DNA detection and serological response in unvaccinated adolescent women",
abstract = "Antibodies directed against the human papillomavirus (HPV) L1 protein are detected in approximately 70{\%} of individuals with HPV infections. The factors associated with a serological response are not characterized. It is hypothesized that the HPV viral load, duration of detection, or both would be associated with seropositivity in adolescent women. Adolescent women (n=117), ages 15-17 at enrolment were followed for a mean of 6.2 years. Quarterly vaginal swabs (mean 22 per participant) were used to identify HPV 6, 11, 16, or 18 DNA (Roche PCR/Linear Array). Type-specific HPV infection was defined as ≥2 positive assays. To approximate viral load, Roche PCR/Linear Array test strips were scored visually based on the strength of signal relative to beta-globin controls. Sera collected near the end of study were tested by cLIA. Regression models were fit to assess associations between strength of signal (as represented by mean and cumulative strength of signal), duration of HPV detection, seropositivity, and serotiter. Detection of HPV DNA was associated with seropositivity for four types combined and for types 6, 16, and 18. Overall, 70.1{\%} of DNA positive episodes were associated with type-specific seropositivity. The cumulative HPV DNA signal strength during periods of HPV detection for types 6, 11, 16, and 18 combined was associated with seropositivity (OR=1.21, 95{\%} CI 1.02-1.44 P=0.026). No other HPV DNA predictors were found to be associated with seropositivity or serotiter.",
keywords = "Adolescent women, Antibody response, Human papillomavirus",
author = "Yan Tong and Aaron Ermel and Wanzhu Tu and Marcia Shew and Darron Brown",
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AU - Tong, Yan

AU - Ermel, Aaron

AU - Tu, Wanzhu

AU - Shew, Marcia

AU - Brown, Darron

PY - 2013/10

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N2 - Antibodies directed against the human papillomavirus (HPV) L1 protein are detected in approximately 70% of individuals with HPV infections. The factors associated with a serological response are not characterized. It is hypothesized that the HPV viral load, duration of detection, or both would be associated with seropositivity in adolescent women. Adolescent women (n=117), ages 15-17 at enrolment were followed for a mean of 6.2 years. Quarterly vaginal swabs (mean 22 per participant) were used to identify HPV 6, 11, 16, or 18 DNA (Roche PCR/Linear Array). Type-specific HPV infection was defined as ≥2 positive assays. To approximate viral load, Roche PCR/Linear Array test strips were scored visually based on the strength of signal relative to beta-globin controls. Sera collected near the end of study were tested by cLIA. Regression models were fit to assess associations between strength of signal (as represented by mean and cumulative strength of signal), duration of HPV detection, seropositivity, and serotiter. Detection of HPV DNA was associated with seropositivity for four types combined and for types 6, 16, and 18. Overall, 70.1% of DNA positive episodes were associated with type-specific seropositivity. The cumulative HPV DNA signal strength during periods of HPV detection for types 6, 11, 16, and 18 combined was associated with seropositivity (OR=1.21, 95% CI 1.02-1.44 P=0.026). No other HPV DNA predictors were found to be associated with seropositivity or serotiter.

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