Association of the protein kinases c-Bcr and Bcr-Abl with proteins of the 14-3-3 family

Gary W. Reuther, Haian Fu, Larry D. Cripe, R. John Collier, Ann Marie Pendergast

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Abstract

In this study, a protein that interacts with sequences encoded by the first exon of the protein kinase Bcr was cloned. The Bcr-associated protein 1 (Bap-1) is a member of the 14-3-3 family of proteins. Bap-1 interacts with full-length c-Bcr and with the chimeric Bcr-Abl tyrosine kinase of Philadelphia chromosome (Ph1)-positive human leukemias. Bap-1 is a substrate for the Bcr serine-threonine kinase and is also phosphorylated on tyrosine by Bcr-Abl but not by c-Abl. Bap-1 may function in the regulation of c-Bcr and may contribute to the transforming activity of Bcr-Abl in vivo. 14-3-3 proteins are essential for cell proliferation and have a role in determining the timing of mitosis in yeast. Through direct binding to sequences present in Bcr and in other proteins implicated in signaling, the mammalian 14-3-3 proteins may link specific signaling protein components to mitogenic and cell-cycle control pathways.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalScience
Volume266
Issue number5182
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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