Association of the sensitivity of mouse granulocyte-macrophage progenitor cells to inhibition by acidic isoferritins with expression of Ia antigens for I-A and I-E/C subregions during DNA synthesis

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Abstract

Murine Ia antigens for the I-A and I-E/C subregions were detected on mouse granulocyte-macrophage progenitor cells (CFU-GM). An association was established betwen the expression of these Ia antigenic determinants on CFU-GM during DNA synthesis and the regulatory action of acidic isoferritins in vitro. Treatment of bone marrow cells of the correct haplotype with monoclonal anti-Ia antibodies for the I-A or I-E/C subregions with complement (C) inhibited colony and cluster formation by ~50%. Similar treatment with monoclonal anti-H-2K antibodies plus C reduced colony and cluster formation by 95 to 100%. The capacity to detect inhibition with anti-Ia plus C depended on the source of conditioned medium used to stimulate colony formation, as well as the source of C used or the cytotoxicity assays. Sources of granulocyte-macrophage colony-stimulatory factors (GM-CSF) containing high levels of inhibitory acidic isoferritins did not allow detection of Ia antigens on CFU-GM. Using GM-CSF free of acidic isoferritins, reduction of colonies and clusters was similar whether bone marrow cells were exposed to anti-Ia plus C, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia antigen-positive CFU-GM were removed, or with anti-Ia plus C or acidic isoferritins after S-phase CFU-GM were removed. Anti-Ia without C did not reduce colony or cluster formation, but did block the inhibitory action of acidic isoferritins, an effect not blocked by monoclonal anti-H-2K antibodies. These results indicate that mouse CFU-GM express Ia during S-phase of the cell cycle, and that it is these Ia-positive cells that are sensitive to regulation by acidic isoferritins.

Original languageEnglish (US)
Pages (from-to)1002-1007
Number of pages6
JournalJournal of Immunology
Volume129
Issue number3
StatePublished - 1982
Externally publishedYes

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Granulocyte-Macrophage Progenitor Cells
Histocompatibility Antigens Class II
DNA
S Phase
Granulocytes
Bone Marrow Cells
Macrophages
acidic isoferritin
Antibodies
Conditioned Culture Medium
Thymidine
Haplotypes
Epitopes
Anti-Idiotypic Antibodies
Cell Cycle
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Association of the sensitivity of mouse granulocyte-macrophage progenitor cells to inhibition by acidic isoferritins with expression of Ia antigens for I-A and I-E/C subregions during DNA synthesis",
abstract = "Murine Ia antigens for the I-A and I-E/C subregions were detected on mouse granulocyte-macrophage progenitor cells (CFU-GM). An association was established betwen the expression of these Ia antigenic determinants on CFU-GM during DNA synthesis and the regulatory action of acidic isoferritins in vitro. Treatment of bone marrow cells of the correct haplotype with monoclonal anti-Ia antibodies for the I-A or I-E/C subregions with complement (C) inhibited colony and cluster formation by ~50{\%}. Similar treatment with monoclonal anti-H-2K antibodies plus C reduced colony and cluster formation by 95 to 100{\%}. The capacity to detect inhibition with anti-Ia plus C depended on the source of conditioned medium used to stimulate colony formation, as well as the source of C used or the cytotoxicity assays. Sources of granulocyte-macrophage colony-stimulatory factors (GM-CSF) containing high levels of inhibitory acidic isoferritins did not allow detection of Ia antigens on CFU-GM. Using GM-CSF free of acidic isoferritins, reduction of colonies and clusters was similar whether bone marrow cells were exposed to anti-Ia plus C, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia antigen-positive CFU-GM were removed, or with anti-Ia plus C or acidic isoferritins after S-phase CFU-GM were removed. Anti-Ia without C did not reduce colony or cluster formation, but did block the inhibitory action of acidic isoferritins, an effect not blocked by monoclonal anti-H-2K antibodies. These results indicate that mouse CFU-GM express Ia during S-phase of the cell cycle, and that it is these Ia-positive cells that are sensitive to regulation by acidic isoferritins.",
author = "Hal Broxmeyer",
year = "1982",
language = "English (US)",
volume = "129",
pages = "1002--1007",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

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T1 - Association of the sensitivity of mouse granulocyte-macrophage progenitor cells to inhibition by acidic isoferritins with expression of Ia antigens for I-A and I-E/C subregions during DNA synthesis

AU - Broxmeyer, Hal

PY - 1982

Y1 - 1982

N2 - Murine Ia antigens for the I-A and I-E/C subregions were detected on mouse granulocyte-macrophage progenitor cells (CFU-GM). An association was established betwen the expression of these Ia antigenic determinants on CFU-GM during DNA synthesis and the regulatory action of acidic isoferritins in vitro. Treatment of bone marrow cells of the correct haplotype with monoclonal anti-Ia antibodies for the I-A or I-E/C subregions with complement (C) inhibited colony and cluster formation by ~50%. Similar treatment with monoclonal anti-H-2K antibodies plus C reduced colony and cluster formation by 95 to 100%. The capacity to detect inhibition with anti-Ia plus C depended on the source of conditioned medium used to stimulate colony formation, as well as the source of C used or the cytotoxicity assays. Sources of granulocyte-macrophage colony-stimulatory factors (GM-CSF) containing high levels of inhibitory acidic isoferritins did not allow detection of Ia antigens on CFU-GM. Using GM-CSF free of acidic isoferritins, reduction of colonies and clusters was similar whether bone marrow cells were exposed to anti-Ia plus C, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia antigen-positive CFU-GM were removed, or with anti-Ia plus C or acidic isoferritins after S-phase CFU-GM were removed. Anti-Ia without C did not reduce colony or cluster formation, but did block the inhibitory action of acidic isoferritins, an effect not blocked by monoclonal anti-H-2K antibodies. These results indicate that mouse CFU-GM express Ia during S-phase of the cell cycle, and that it is these Ia-positive cells that are sensitive to regulation by acidic isoferritins.

AB - Murine Ia antigens for the I-A and I-E/C subregions were detected on mouse granulocyte-macrophage progenitor cells (CFU-GM). An association was established betwen the expression of these Ia antigenic determinants on CFU-GM during DNA synthesis and the regulatory action of acidic isoferritins in vitro. Treatment of bone marrow cells of the correct haplotype with monoclonal anti-Ia antibodies for the I-A or I-E/C subregions with complement (C) inhibited colony and cluster formation by ~50%. Similar treatment with monoclonal anti-H-2K antibodies plus C reduced colony and cluster formation by 95 to 100%. The capacity to detect inhibition with anti-Ia plus C depended on the source of conditioned medium used to stimulate colony formation, as well as the source of C used or the cytotoxicity assays. Sources of granulocyte-macrophage colony-stimulatory factors (GM-CSF) containing high levels of inhibitory acidic isoferritins did not allow detection of Ia antigens on CFU-GM. Using GM-CSF free of acidic isoferritins, reduction of colonies and clusters was similar whether bone marrow cells were exposed to anti-Ia plus C, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia antigen-positive CFU-GM were removed, or with anti-Ia plus C or acidic isoferritins after S-phase CFU-GM were removed. Anti-Ia without C did not reduce colony or cluster formation, but did block the inhibitory action of acidic isoferritins, an effect not blocked by monoclonal anti-H-2K antibodies. These results indicate that mouse CFU-GM express Ia during S-phase of the cell cycle, and that it is these Ia-positive cells that are sensitive to regulation by acidic isoferritins.

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