Association of UCP-3 rs1626521 with obesity and stomach functions in humans

Andres Acosta, Michael Camilleri, Andrea Shin, Maria I. Vazquez-Roque, Johanna Iturrino, Ian R. Lanza, K. Sreekumaran Nair, Duane Burton, Jessica O'Neill, Deborah Eckert, Paula Carlson, Adrian Vella, Alan R. Zinsmeister

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective To examine the association of gene variants of uncoupling proteins (UCP)-2 and -3 with obesity and gastrointestinal (GI) traits. Methods In 255 overweight or obese adults, the associations of gene variants in UCP-2 (-3474, rs659366) and UCP-3 (rs1626521, rs2075577, rs15763) with body weight (BW) and GI traits were studied. Gene variants were genotyped by TaqMan® assay. The associations of genotypes with BW and GI traits (gastric emptying, gastric volume, satiety by buffet meal, satiation by nutrient drink test and GI hormones) were assessed using ANOVA corrected for false detection rate (FDR). Results A novel UCP-3 gene variant, rs1626521, was identified; it was associated with BW (P = 0.039), waist circumference (P = 0.035), and significantly higher postprandial gastric volume (P = 0.003) and calories ingested at buffet meal (P = 0.006, both significant with FDR). In a subgroup of 11 participants, rs1626521 was also associated with reduced mitochondrial bioenergetics efficiency in skeletal muscle (P = 0.051). In an in vitro study in HEK293 cells, rs1626521 reduced UCP-3 protein expression (P = 0.049). Associations detected between other genotypes and GI traits were nonsignificant with FDR. Conclusions A newly identified functional variant (rs1626521) in UCP-3 affects postprandial gastric functions and satiety and may contribute to weight gain and alter human mitochondrial function.

Original languageEnglish (US)
Pages (from-to)898-906
Number of pages9
JournalObesity
Volume23
Issue number4
DOIs
StatePublished - Apr 1 2015

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Stomach
Obesity
Body Weight
Genes
Meals
Genotype
Satiation
Gastrointestinal Hormones
HEK293 Cells
Gastric Emptying
Waist Circumference
Energy Metabolism
Weight Gain
Analysis of Variance
Skeletal Muscle
Uncoupling Protein 3
Food
Proteins
Uncoupling Protein 2

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Acosta, A., Camilleri, M., Shin, A., Vazquez-Roque, M. I., Iturrino, J., Lanza, I. R., ... Zinsmeister, A. R. (2015). Association of UCP-3 rs1626521 with obesity and stomach functions in humans. Obesity, 23(4), 898-906. https://doi.org/10.1002/oby.21039

Association of UCP-3 rs1626521 with obesity and stomach functions in humans. / Acosta, Andres; Camilleri, Michael; Shin, Andrea; Vazquez-Roque, Maria I.; Iturrino, Johanna; Lanza, Ian R.; Nair, K. Sreekumaran; Burton, Duane; O'Neill, Jessica; Eckert, Deborah; Carlson, Paula; Vella, Adrian; Zinsmeister, Alan R.

In: Obesity, Vol. 23, No. 4, 01.04.2015, p. 898-906.

Research output: Contribution to journalArticle

Acosta, A, Camilleri, M, Shin, A, Vazquez-Roque, MI, Iturrino, J, Lanza, IR, Nair, KS, Burton, D, O'Neill, J, Eckert, D, Carlson, P, Vella, A & Zinsmeister, AR 2015, 'Association of UCP-3 rs1626521 with obesity and stomach functions in humans', Obesity, vol. 23, no. 4, pp. 898-906. https://doi.org/10.1002/oby.21039
Acosta A, Camilleri M, Shin A, Vazquez-Roque MI, Iturrino J, Lanza IR et al. Association of UCP-3 rs1626521 with obesity and stomach functions in humans. Obesity. 2015 Apr 1;23(4):898-906. https://doi.org/10.1002/oby.21039
Acosta, Andres ; Camilleri, Michael ; Shin, Andrea ; Vazquez-Roque, Maria I. ; Iturrino, Johanna ; Lanza, Ian R. ; Nair, K. Sreekumaran ; Burton, Duane ; O'Neill, Jessica ; Eckert, Deborah ; Carlson, Paula ; Vella, Adrian ; Zinsmeister, Alan R. / Association of UCP-3 rs1626521 with obesity and stomach functions in humans. In: Obesity. 2015 ; Vol. 23, No. 4. pp. 898-906.
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abstract = "Objective To examine the association of gene variants of uncoupling proteins (UCP)-2 and -3 with obesity and gastrointestinal (GI) traits. Methods In 255 overweight or obese adults, the associations of gene variants in UCP-2 (-3474, rs659366) and UCP-3 (rs1626521, rs2075577, rs15763) with body weight (BW) and GI traits were studied. Gene variants were genotyped by TaqMan{\circledR} assay. The associations of genotypes with BW and GI traits (gastric emptying, gastric volume, satiety by buffet meal, satiation by nutrient drink test and GI hormones) were assessed using ANOVA corrected for false detection rate (FDR). Results A novel UCP-3 gene variant, rs1626521, was identified; it was associated with BW (P = 0.039), waist circumference (P = 0.035), and significantly higher postprandial gastric volume (P = 0.003) and calories ingested at buffet meal (P = 0.006, both significant with FDR). In a subgroup of 11 participants, rs1626521 was also associated with reduced mitochondrial bioenergetics efficiency in skeletal muscle (P = 0.051). In an in vitro study in HEK293 cells, rs1626521 reduced UCP-3 protein expression (P = 0.049). Associations detected between other genotypes and GI traits were nonsignificant with FDR. Conclusions A newly identified functional variant (rs1626521) in UCP-3 affects postprandial gastric functions and satiety and may contribute to weight gain and alter human mitochondrial function.",
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AU - Iturrino, Johanna

AU - Lanza, Ian R.

AU - Nair, K. Sreekumaran

AU - Burton, Duane

AU - O'Neill, Jessica

AU - Eckert, Deborah

AU - Carlson, Paula

AU - Vella, Adrian

AU - Zinsmeister, Alan R.

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N2 - Objective To examine the association of gene variants of uncoupling proteins (UCP)-2 and -3 with obesity and gastrointestinal (GI) traits. Methods In 255 overweight or obese adults, the associations of gene variants in UCP-2 (-3474, rs659366) and UCP-3 (rs1626521, rs2075577, rs15763) with body weight (BW) and GI traits were studied. Gene variants were genotyped by TaqMan® assay. The associations of genotypes with BW and GI traits (gastric emptying, gastric volume, satiety by buffet meal, satiation by nutrient drink test and GI hormones) were assessed using ANOVA corrected for false detection rate (FDR). Results A novel UCP-3 gene variant, rs1626521, was identified; it was associated with BW (P = 0.039), waist circumference (P = 0.035), and significantly higher postprandial gastric volume (P = 0.003) and calories ingested at buffet meal (P = 0.006, both significant with FDR). In a subgroup of 11 participants, rs1626521 was also associated with reduced mitochondrial bioenergetics efficiency in skeletal muscle (P = 0.051). In an in vitro study in HEK293 cells, rs1626521 reduced UCP-3 protein expression (P = 0.049). Associations detected between other genotypes and GI traits were nonsignificant with FDR. Conclusions A newly identified functional variant (rs1626521) in UCP-3 affects postprandial gastric functions and satiety and may contribute to weight gain and alter human mitochondrial function.

AB - Objective To examine the association of gene variants of uncoupling proteins (UCP)-2 and -3 with obesity and gastrointestinal (GI) traits. Methods In 255 overweight or obese adults, the associations of gene variants in UCP-2 (-3474, rs659366) and UCP-3 (rs1626521, rs2075577, rs15763) with body weight (BW) and GI traits were studied. Gene variants were genotyped by TaqMan® assay. The associations of genotypes with BW and GI traits (gastric emptying, gastric volume, satiety by buffet meal, satiation by nutrient drink test and GI hormones) were assessed using ANOVA corrected for false detection rate (FDR). Results A novel UCP-3 gene variant, rs1626521, was identified; it was associated with BW (P = 0.039), waist circumference (P = 0.035), and significantly higher postprandial gastric volume (P = 0.003) and calories ingested at buffet meal (P = 0.006, both significant with FDR). In a subgroup of 11 participants, rs1626521 was also associated with reduced mitochondrial bioenergetics efficiency in skeletal muscle (P = 0.051). In an in vitro study in HEK293 cells, rs1626521 reduced UCP-3 protein expression (P = 0.049). Associations detected between other genotypes and GI traits were nonsignificant with FDR. Conclusions A newly identified functional variant (rs1626521) in UCP-3 affects postprandial gastric functions and satiety and may contribute to weight gain and alter human mitochondrial function.

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