Associations between hippocampal morphometry and neuropathologic markers of Alzheimer's disease using 7 T MRI

Anna E. Blanken, Sona Hurtz, Chris Zarow, Kristina Biado, Hedieh Honarpisheh, Johanne Somme, Jenny Brook, Spencer Tung, Emily Kraft, Darrick Lo, Denise W. Ng, Harry V. Vinters, Liana G. Apostolova

Research output: Contribution to journalArticle

12 Scopus citations


Hippocampal atrophy, amyloid plaques, and neurofibrillary tangles are established pathologic markers of Alzheimer's disease. We analyzed the temporal lobes of 9 Alzheimer's dementia (AD) and 7 cognitively normal (NC) subjects. Brains were scanned post-mortem at 7 Tesla. We extracted hippocampal volumes and radial distances using automated segmentation techniques. Hippocampal slices were stained for amyloid beta (Aβ), tau, and cresyl violet to evaluate neuronal counts. The hippocampal subfields, CA1, CA2, CA3, CA4, and subiculum were manually traced so that the neuronal counts, Aβ, and tau burden could be obtained for each region. We used linear regression to detect associations between hippocampal atrophy in 3D, clinical diagnosis and total as well as subfield pathology burden measures. As expected, we found significant correlations between hippocampal radial distance and mean neuronal count, as well as diagnosis. There were subfield specific associations between hippocampal radial distance and tau in CA2, and cresyl violet neuronal counts in CA1 and subiculum. These results provide further validation for the European Alzheimer's Disease Consortium Alzheimer's Disease Neuroimaging Initiative Center Harmonized Hippocampal Segmentation Protocol (HarP).

Original languageEnglish (US)
Pages (from-to)56-61
Number of pages6
JournalNeuroImage: Clinical
StatePublished - Jan 1 2017



  • Alzheimer's disease
  • Brain atrophy
  • Hippocampus
  • Imaging
  • MRI
  • Neuropathology

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Neurology
  • Clinical Neurology
  • Cognitive Neuroscience

Cite this

Blanken, A. E., Hurtz, S., Zarow, C., Biado, K., Honarpisheh, H., Somme, J., Brook, J., Tung, S., Kraft, E., Lo, D., Ng, D. W., Vinters, H. V., & Apostolova, L. G. (2017). Associations between hippocampal morphometry and neuropathologic markers of Alzheimer's disease using 7 T MRI. NeuroImage: Clinical, 15, 56-61.