Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels as a central target for the development of human AD and AD-like pathology in aged transgenic mice

Gjumrakch Aliev, Dilara Seyidova, Maxwell L. Neal, Jiong Shi, Bruce Lamb, Sandra L. Siedlak, Harry V. Vinters, Elizabeth Head, George Perry, Joseph C. Lamanna, Robert P. Friedland, Carl W. Cotman

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Abstract

We have studied the ultrastructural features of vascular lesions and mitochondria in brain vascular wall cells from human AD brain biopsy, human short postmortem brain tissues, and yeast artificial chromosome (YAC) and C57B6/SJL transgenic positive (Tg+) mice overexpressing amyloid beta precursor protein (AβPP). In situ hybridization using mitochondrial DNA (mtDNA) probes for human wild type, 5 kb deleted, and mouse mtDNA was performed, along with immunocytochemistry using antibodies against amyloid precursor protein (APP), 8-hydroxy-2′-guanosine (8-OHG), and cytochrome c oxidase (COX). There was a higher degree of amyloid deposition in the vascular walls of the human AD, YAC, and C57B6/SJL Tg (+) mice compared to age-matched controls. In addition, vessels with more severe lesions showed immunopositive staining for APP and possessed large, lipid-laden vacuoles in the cytoplasm of endothelial cells (EC). Significantly more mitochondrial abnormalities were seen in human AD, YAC, and C57B6/SJL Tg (+) mouse micro-vessels where lesions occurred. In situ hybridization using wild and chimera (5 kb) mtDNA probes revealed positive signals in damaged mitochondria from the vascular endothelium and in perivascular cells of lesioned microvessels close to regions of large amyloid deposition. These features were absent in undamaged regions of human AD tissues, YAC and C57B6/SJL Tg (+) mouse tissues, and in age-matched control subjects. In addition, vessels with atherosclerotic lesions revealed endothelium and perivascular cells possessing clusters of wild and deleted mtDNA positive probes. These mtDNA deletions were accompanied by increased amounts of immunoreactive APP, 8-OHG, and COX in the same cellular compartment. Our observations demonstrate that vascular wall cells, especially their mitochondria, appear to be a central target for oxidative stress-induced damage.

Original languageEnglish (US)
Pages (from-to)45-64
Number of pages20
JournalAnnals of the New York Academy of Sciences
Volume977
StatePublished - 2002
Externally publishedYes

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Mitochondria
Human Development
Pathology
Microvessels
Mitochondrial DNA
Yeast Artificial Chromosomes
Amyloid beta-Protein Precursor
Transgenic Mice
Brain
Chromosomes
Yeast
Blood Vessels
DNA
DNA Probes
Tissue
Amyloid
Cell Wall
In Situ Hybridization
Oxidative stress
Biopsy

Keywords

  • Alzheimer's disease
  • Free radicals
  • Metabolism
  • Mitochondria
  • Neurodegeneration
  • Oxidative stress
  • Vascular factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels as a central target for the development of human AD and AD-like pathology in aged transgenic mice. / Aliev, Gjumrakch; Seyidova, Dilara; Neal, Maxwell L.; Shi, Jiong; Lamb, Bruce; Siedlak, Sandra L.; Vinters, Harry V.; Head, Elizabeth; Perry, George; Lamanna, Joseph C.; Friedland, Robert P.; Cotman, Carl W.

In: Annals of the New York Academy of Sciences, Vol. 977, 2002, p. 45-64.

Research output: Contribution to journalArticle

Aliev, G, Seyidova, D, Neal, ML, Shi, J, Lamb, B, Siedlak, SL, Vinters, HV, Head, E, Perry, G, Lamanna, JC, Friedland, RP & Cotman, CW 2002, 'Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels as a central target for the development of human AD and AD-like pathology in aged transgenic mice', Annals of the New York Academy of Sciences, vol. 977, pp. 45-64.
Aliev, Gjumrakch ; Seyidova, Dilara ; Neal, Maxwell L. ; Shi, Jiong ; Lamb, Bruce ; Siedlak, Sandra L. ; Vinters, Harry V. ; Head, Elizabeth ; Perry, George ; Lamanna, Joseph C. ; Friedland, Robert P. ; Cotman, Carl W. / Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels as a central target for the development of human AD and AD-like pathology in aged transgenic mice. In: Annals of the New York Academy of Sciences. 2002 ; Vol. 977. pp. 45-64.
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abstract = "We have studied the ultrastructural features of vascular lesions and mitochondria in brain vascular wall cells from human AD brain biopsy, human short postmortem brain tissues, and yeast artificial chromosome (YAC) and C57B6/SJL transgenic positive (Tg+) mice overexpressing amyloid beta precursor protein (AβPP). In situ hybridization using mitochondrial DNA (mtDNA) probes for human wild type, 5 kb deleted, and mouse mtDNA was performed, along with immunocytochemistry using antibodies against amyloid precursor protein (APP), 8-hydroxy-2′-guanosine (8-OHG), and cytochrome c oxidase (COX). There was a higher degree of amyloid deposition in the vascular walls of the human AD, YAC, and C57B6/SJL Tg (+) mice compared to age-matched controls. In addition, vessels with more severe lesions showed immunopositive staining for APP and possessed large, lipid-laden vacuoles in the cytoplasm of endothelial cells (EC). Significantly more mitochondrial abnormalities were seen in human AD, YAC, and C57B6/SJL Tg (+) mouse micro-vessels where lesions occurred. In situ hybridization using wild and chimera (5 kb) mtDNA probes revealed positive signals in damaged mitochondria from the vascular endothelium and in perivascular cells of lesioned microvessels close to regions of large amyloid deposition. These features were absent in undamaged regions of human AD tissues, YAC and C57B6/SJL Tg (+) mouse tissues, and in age-matched control subjects. In addition, vessels with atherosclerotic lesions revealed endothelium and perivascular cells possessing clusters of wild and deleted mtDNA positive probes. These mtDNA deletions were accompanied by increased amounts of immunoreactive APP, 8-OHG, and COX in the same cellular compartment. Our observations demonstrate that vascular wall cells, especially their mitochondria, appear to be a central target for oxidative stress-induced damage.",
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T1 - Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels as a central target for the development of human AD and AD-like pathology in aged transgenic mice

AU - Aliev, Gjumrakch

AU - Seyidova, Dilara

AU - Neal, Maxwell L.

AU - Shi, Jiong

AU - Lamb, Bruce

AU - Siedlak, Sandra L.

AU - Vinters, Harry V.

AU - Head, Elizabeth

AU - Perry, George

AU - Lamanna, Joseph C.

AU - Friedland, Robert P.

AU - Cotman, Carl W.

PY - 2002

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AB - We have studied the ultrastructural features of vascular lesions and mitochondria in brain vascular wall cells from human AD brain biopsy, human short postmortem brain tissues, and yeast artificial chromosome (YAC) and C57B6/SJL transgenic positive (Tg+) mice overexpressing amyloid beta precursor protein (AβPP). In situ hybridization using mitochondrial DNA (mtDNA) probes for human wild type, 5 kb deleted, and mouse mtDNA was performed, along with immunocytochemistry using antibodies against amyloid precursor protein (APP), 8-hydroxy-2′-guanosine (8-OHG), and cytochrome c oxidase (COX). There was a higher degree of amyloid deposition in the vascular walls of the human AD, YAC, and C57B6/SJL Tg (+) mice compared to age-matched controls. In addition, vessels with more severe lesions showed immunopositive staining for APP and possessed large, lipid-laden vacuoles in the cytoplasm of endothelial cells (EC). Significantly more mitochondrial abnormalities were seen in human AD, YAC, and C57B6/SJL Tg (+) mouse micro-vessels where lesions occurred. In situ hybridization using wild and chimera (5 kb) mtDNA probes revealed positive signals in damaged mitochondria from the vascular endothelium and in perivascular cells of lesioned microvessels close to regions of large amyloid deposition. These features were absent in undamaged regions of human AD tissues, YAC and C57B6/SJL Tg (+) mouse tissues, and in age-matched control subjects. In addition, vessels with atherosclerotic lesions revealed endothelium and perivascular cells possessing clusters of wild and deleted mtDNA positive probes. These mtDNA deletions were accompanied by increased amounts of immunoreactive APP, 8-OHG, and COX in the same cellular compartment. Our observations demonstrate that vascular wall cells, especially their mitochondria, appear to be a central target for oxidative stress-induced damage.

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KW - Metabolism

KW - Mitochondria

KW - Neurodegeneration

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