Atomic force microscopy of DNA and bacteriophage in air, water and propanol: The role of adhesion forces

Y. L. Lyubchenko, P. I. Oden, D. Lampner, S. M. Lindsay, K. A. Dunker

Research output: Contribution to journalArticlepeer-review

151 Scopus citations

Abstract

We have developed a chemical treatment for the mica surface which allows blopolymers to be held in place for atomic force microscopy, even under water, using conventional, untreated force sensing tips. We illustrate the procedure with images of σ DNA and fd phage. The phage adheres well enough to permit in situ imaging of the adsorption process in water. These experiments yield a mean length for the phage of 883±72nm. This compares with a measured length of 883±33nm when the phage are imaged after drying following adsorption from water, showing that the effect of dehydration is quite small. Adhesion forces between the force sensing tip and the substrate and the sensing tip and the blomolecules are very different in the three media (air, water and propanol). The apparent height of the phage and the width and height of the DNA depends upon these adhesion forces quite strongly. In contrast, changing the Hookean spring force exerted by the scanning tip makes little difference. These results suggest that the chemical factors involved in adhesion can dominate atomic force images and that the composition of the scanning tip is at least as important a factor as its geometry.

Original languageEnglish (US)
Pages (from-to)1117-1123
Number of pages7
JournalNucleic acids research
Volume21
Issue number5
DOIs
StatePublished - Mar 11 1993

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Atomic force microscopy of DNA and bacteriophage in air, water and propanol: The role of adhesion forces'. Together they form a unique fingerprint.

Cite this