ATP-dependent K+ channels contribute to local metabolic coronary vasodilation in experimental diabetes

Johnathan Tune, Clement Yeh, Srinath Setty, H. Fred Downey

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study tested whether ATP-dependent K+ channels (KATP channels) are an important mechanism of functional coronary hyperemia in conscious, instrument-implanted diabetic dogs. Data were collected at rest and during exercise before and after induction of diabetes with alloxan monohydrate (40-60 mg/kg intravenously). KATP channels were inhibited with glibenclamide (1 mg/kg intravenously). In nondiabetic dogs, arterial plasma glucose concentration increased from 4.8 ± 0.3 to 21.5 ± 2.2 mmol/l 1 week after alloxan injection. In nondiabetic dogs, exercise increased myocardial oxygen consumption (MVo2) 3.4-fold, myocardial O2 delivery 3.0-fold, and heart rate 2.4-fold. Coronary venous Po2 decreased from 19.9 ± 0.8 mmHg at rest to 14.8 ± 0.8 mmHg during exercise. Diabetes significantly reduced myocardial 02 delivery and lowered coronary venous Po2 from 16.3 ± 0.6 mmHg at rest to 13.1 ± 0.9 mmHg during exercise. Glibenclamide did not alter the slope of the coronary venous PO2 versus MVo2 relationship in nondiabetic dogs. In diabetic dogs, however, glibenclamide further reduced myocardial O2 delivery; coronary venous Po2 fell to 9.0 ± 1.0 mmHg during exercise, and the slope of the coronary venous PO2 versus MVo2 relationship steepened. These findings indicate that KATP channels contribute to local metabolic coronary vasodilation in alloxan-induced diabetic dogs.

Original languageEnglish (US)
Pages (from-to)1201-1207
Number of pages7
JournalDiabetes
Volume51
Issue number4
StatePublished - 2002
Externally publishedYes

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Vasodilation
Adenosine Triphosphate
Dogs
KATP Channels
Glyburide
Alloxan
Experimental Diabetes Mellitus
Hyperemia
Oxygen Consumption
Heart Rate
Glucose
Injections

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

ATP-dependent K+ channels contribute to local metabolic coronary vasodilation in experimental diabetes. / Tune, Johnathan; Yeh, Clement; Setty, Srinath; Fred Downey, H.

In: Diabetes, Vol. 51, No. 4, 2002, p. 1201-1207.

Research output: Contribution to journalArticle

Tune, J, Yeh, C, Setty, S & Fred Downey, H 2002, 'ATP-dependent K+ channels contribute to local metabolic coronary vasodilation in experimental diabetes', Diabetes, vol. 51, no. 4, pp. 1201-1207.
Tune, Johnathan ; Yeh, Clement ; Setty, Srinath ; Fred Downey, H. / ATP-dependent K+ channels contribute to local metabolic coronary vasodilation in experimental diabetes. In: Diabetes. 2002 ; Vol. 51, No. 4. pp. 1201-1207.
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