ATP depletion alters myosin Iβ cellular location in LLC-PK1 cells

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Abstract

The brush border (BB) of the proximal tubule cell (PTC) requires dynamic membrane events for function. The actin cytoskeleton is necessary for structure and function in this region. ATP depletion disrupts both structure and function. In this report, myosin Iβ location in LLC-PK1 cells was followed during ATP depletion and repletion using immunofluorescence and Western blot techniques. Myosin Iβ colocalized with F-actin in the microvilli and cell periphery, but no colocalization was observed with stress fibers. ATP depletion increased the apical F-actin, and myosin Iβ was colocalized there. In addition, after ATP depletion, myosin Iβ was extracted less by Triton X-100. These changes were reversed after ATP repletion. Finally, immunofluorescence of kidney sections shows myosin Iβ in the BB. These results place this motor in a dynamic region of the PTC where its actin and membrane binding domains can contribute to PTC function.

Original languageEnglish (US)
Pages (from-to)C1680-C1690
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number5 41-5
StatePublished - Jun 26 1997

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Keywords

  • actin cytoskeleton
  • brush border
  • motor
  • myosin I
  • proximal tubule cell

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

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