Attenuated Ca2+ response to acetylcholine in endothelial cells from aorta of aldosterone-salt hypertensive rats

Y. Liu, A. W. Jones, Michael Sturek

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ca2+ is essential for endothelial production of vasorelaxing factors. We determined whether the impaired endothelium-dependent relaxation in aldosterone-salt hypertensive rats (AHR) is associated with a decreased free Ca2+ ([Ca2+](i)) response in endothelial cells. In isolated aorta, the EC50 for the acetylcholine-induced endothelium-dependent relaxations did not differ between AHR and the age-matched control-salt rats (CSR). However, maximal relaxation was significantly reduced by 47% in AHR (P 2+](i) was measured with fura-2 microfluorometry in endothelial cells freshly dispersed from aorta. Although the basal [Ca2+](i) was not different between CSR and AHR, the peak [Ca2+](i) response to acetylcholine wag significantly reduced in cells from AHR compared with CSR (P 2+](i) responses to acetylcholine may be involved in the impaired endothelium-dependent relaxation in aorta from AHR.

Original languageEnglish (US)
Pages (from-to)404-408
Number of pages5
JournalAmerican Journal of Hypertension
Volume8
Issue number4 I
StatePublished - 1995
Externally publishedYes

Fingerprint

Aldosterone
Acetylcholine
Aorta
Endothelial Cells
Salts
Endothelium
Cytophotometry
Fura-2

Keywords

  • Endothelium-dependent relaxation
  • Fura-2
  • Indomethacin
  • N(G)-nitro L-arginine methyl ester
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Attenuated Ca2+ response to acetylcholine in endothelial cells from aorta of aldosterone-salt hypertensive rats. / Liu, Y.; Jones, A. W.; Sturek, Michael.

In: American Journal of Hypertension, Vol. 8, No. 4 I, 1995, p. 404-408.

Research output: Contribution to journalArticle

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