Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney

Jinhwan Lim, Ruth A. Sanders, Ronnie L. Yeager, Deborah S. Millsap, John B. Watkins, Janis T. Eells, Diane S. Henshel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a potent developmental teratogen inducing oxidative stress and sublethal changes in multiple organs, provokes developmental renal injuries. In this study, we investigated TCDD-induced biochemical changes and the therapeutic efficacy of photobiomodulation (670 nm; 4 J/cm2) on oxidative stress in chicken kidneys during development. Eggs were injected once prior to incubation with TCDD (2 pg/g or 200 pg/g) or sunflower oil vehicle control. Half of the eggs in each dose group were then treated with red light once per day through embryonic day 20 (E20). Upon hatching at E21, the kidneys were collected and assayed for glutathione peroxidase, glutathione reductase, catalase, superoxide dimutase, and glutathione-S-transferase activities, as well as reduced glutathione and ATP levels, and lipid peroxidation. TCDD exposure alone suppressed the activity of the antioxidant enzymes, increased lipid peroxidation, and depleted available ATP. The biochemical indicators of oxidative and energy stress in the kidney were reversed by daily phototherapy, restoring ATP and glutathione contents and increasing antioxidant enzyme activities to control levels. Photobiomodulation also normalized the level of lipid peroxidation increased by TCDD exposure. The results of this study suggest that 670 nm photobiomodulation may be useful as a noninvasive treatment for renal injury resulting from chemically induced cellular oxidative and energy stress.

Original languageEnglish
Pages (from-to)230-239
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume22
Issue number4
DOIs
StatePublished - 2008

Fingerprint

Oxidative stress
Chickens
Oxidative Stress
Kidney
Lipid Peroxidation
Adenosine Triphosphate
Lipids
Eggs
Glutathione
Antioxidants
Teratogens
Phototherapy
Glutathione Reductase
Level control
Wounds and Injuries
Enzyme activity
Enzymes
Glutathione Peroxidase
Glutathione Transferase
Superoxides

Keywords

  • Antioxidant enzymes
  • Kidney
  • Oxidative stress
  • Photobiomodulation
  • TCDD

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Lim, J., Sanders, R. A., Yeager, R. L., Millsap, D. S., Watkins, J. B., Eells, J. T., & Henshel, D. S. (2008). Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney. Journal of Biochemical and Molecular Toxicology, 22(4), 230-239. https://doi.org/10.1002/jbt.20233

Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney. / Lim, Jinhwan; Sanders, Ruth A.; Yeager, Ronnie L.; Millsap, Deborah S.; Watkins, John B.; Eells, Janis T.; Henshel, Diane S.

In: Journal of Biochemical and Molecular Toxicology, Vol. 22, No. 4, 2008, p. 230-239.

Research output: Contribution to journalArticle

Lim, J, Sanders, RA, Yeager, RL, Millsap, DS, Watkins, JB, Eells, JT & Henshel, DS 2008, 'Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney', Journal of Biochemical and Molecular Toxicology, vol. 22, no. 4, pp. 230-239. https://doi.org/10.1002/jbt.20233
Lim, Jinhwan ; Sanders, Ruth A. ; Yeager, Ronnie L. ; Millsap, Deborah S. ; Watkins, John B. ; Eells, Janis T. ; Henshel, Diane S. / Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney. In: Journal of Biochemical and Molecular Toxicology. 2008 ; Vol. 22, No. 4. pp. 230-239.
@article{1bf764d875f74735af47d55694b44b6a,
title = "Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney",
abstract = "2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a potent developmental teratogen inducing oxidative stress and sublethal changes in multiple organs, provokes developmental renal injuries. In this study, we investigated TCDD-induced biochemical changes and the therapeutic efficacy of photobiomodulation (670 nm; 4 J/cm2) on oxidative stress in chicken kidneys during development. Eggs were injected once prior to incubation with TCDD (2 pg/g or 200 pg/g) or sunflower oil vehicle control. Half of the eggs in each dose group were then treated with red light once per day through embryonic day 20 (E20). Upon hatching at E21, the kidneys were collected and assayed for glutathione peroxidase, glutathione reductase, catalase, superoxide dimutase, and glutathione-S-transferase activities, as well as reduced glutathione and ATP levels, and lipid peroxidation. TCDD exposure alone suppressed the activity of the antioxidant enzymes, increased lipid peroxidation, and depleted available ATP. The biochemical indicators of oxidative and energy stress in the kidney were reversed by daily phototherapy, restoring ATP and glutathione contents and increasing antioxidant enzyme activities to control levels. Photobiomodulation also normalized the level of lipid peroxidation increased by TCDD exposure. The results of this study suggest that 670 nm photobiomodulation may be useful as a noninvasive treatment for renal injury resulting from chemically induced cellular oxidative and energy stress.",
keywords = "Antioxidant enzymes, Kidney, Oxidative stress, Photobiomodulation, TCDD",
author = "Jinhwan Lim and Sanders, {Ruth A.} and Yeager, {Ronnie L.} and Millsap, {Deborah S.} and Watkins, {John B.} and Eells, {Janis T.} and Henshel, {Diane S.}",
year = "2008",
doi = "10.1002/jbt.20233",
language = "English",
volume = "22",
pages = "230--239",
journal = "Journal of Biochemical and Molecular Toxicology",
issn = "1095-6670",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney

AU - Lim, Jinhwan

AU - Sanders, Ruth A.

AU - Yeager, Ronnie L.

AU - Millsap, Deborah S.

AU - Watkins, John B.

AU - Eells, Janis T.

AU - Henshel, Diane S.

PY - 2008

Y1 - 2008

N2 - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a potent developmental teratogen inducing oxidative stress and sublethal changes in multiple organs, provokes developmental renal injuries. In this study, we investigated TCDD-induced biochemical changes and the therapeutic efficacy of photobiomodulation (670 nm; 4 J/cm2) on oxidative stress in chicken kidneys during development. Eggs were injected once prior to incubation with TCDD (2 pg/g or 200 pg/g) or sunflower oil vehicle control. Half of the eggs in each dose group were then treated with red light once per day through embryonic day 20 (E20). Upon hatching at E21, the kidneys were collected and assayed for glutathione peroxidase, glutathione reductase, catalase, superoxide dimutase, and glutathione-S-transferase activities, as well as reduced glutathione and ATP levels, and lipid peroxidation. TCDD exposure alone suppressed the activity of the antioxidant enzymes, increased lipid peroxidation, and depleted available ATP. The biochemical indicators of oxidative and energy stress in the kidney were reversed by daily phototherapy, restoring ATP and glutathione contents and increasing antioxidant enzyme activities to control levels. Photobiomodulation also normalized the level of lipid peroxidation increased by TCDD exposure. The results of this study suggest that 670 nm photobiomodulation may be useful as a noninvasive treatment for renal injury resulting from chemically induced cellular oxidative and energy stress.

AB - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a potent developmental teratogen inducing oxidative stress and sublethal changes in multiple organs, provokes developmental renal injuries. In this study, we investigated TCDD-induced biochemical changes and the therapeutic efficacy of photobiomodulation (670 nm; 4 J/cm2) on oxidative stress in chicken kidneys during development. Eggs were injected once prior to incubation with TCDD (2 pg/g or 200 pg/g) or sunflower oil vehicle control. Half of the eggs in each dose group were then treated with red light once per day through embryonic day 20 (E20). Upon hatching at E21, the kidneys were collected and assayed for glutathione peroxidase, glutathione reductase, catalase, superoxide dimutase, and glutathione-S-transferase activities, as well as reduced glutathione and ATP levels, and lipid peroxidation. TCDD exposure alone suppressed the activity of the antioxidant enzymes, increased lipid peroxidation, and depleted available ATP. The biochemical indicators of oxidative and energy stress in the kidney were reversed by daily phototherapy, restoring ATP and glutathione contents and increasing antioxidant enzyme activities to control levels. Photobiomodulation also normalized the level of lipid peroxidation increased by TCDD exposure. The results of this study suggest that 670 nm photobiomodulation may be useful as a noninvasive treatment for renal injury resulting from chemically induced cellular oxidative and energy stress.

KW - Antioxidant enzymes

KW - Kidney

KW - Oxidative stress

KW - Photobiomodulation

KW - TCDD

UR - http://www.scopus.com/inward/record.url?scp=50949109339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50949109339&partnerID=8YFLogxK

U2 - 10.1002/jbt.20233

DO - 10.1002/jbt.20233

M3 - Article

C2 - 18752309

AN - SCOPUS:50949109339

VL - 22

SP - 230

EP - 239

JO - Journal of Biochemical and Molecular Toxicology

JF - Journal of Biochemical and Molecular Toxicology

SN - 1095-6670

IS - 4

ER -