Augmentation of UVB radiation-mediated early gene expression by the epidermal platelet-activating factor receptor

Jeffrey Travers, Howard Edenberg, Qiwei Zhang, Mohammed Al-Hassani, Qiaofang Yi, Sulochanadevi Baskaran, Raymond Konger

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

UVB radiation (UVB) is a known inducer of many biological changes in human skin, and triggers the production of glycerophosphocholines that act as platelet-activating factor (PAF) agonists. To gain a better insight into the role of the epidermal PAF receptor (PAF-R) in UVB-mediated gene expression, Affymetrix oligonucleotide microarrays were used to compare mRNA expression in the PAF-R-negative epithelial cell line KB-expressing PAF-Rs (KBP) with that in KB cells transduced with a vector control (KBM). Total RNA was isolated from KB cells 1 hour after treatment with a PAF-R agonist or UVB irradiation. Treatment of KBP with PAF agonist resulted in altered expression of 220 genes, including cytokines and growth factors. UVB irradiation of KB cells resulted in an increased expression of genes in both cell types. A panel of genes including cytokines CCL20 (MIP3α) and tumor necrosis factor-α (TNF-α) were upregulated selectively in KBP cells and are also selectively upregulated in response to PAF agonist. Consistent with these in vitro findings, UVB irradiation resulted in increased levels of epidermal CCL20 and TNF-α mRNA in wild-type over PAF-R-deficient mice in vivo. These studies provide evidence that the epidermal PAF-R can modulate UVB-mediated early gene expression.

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalJournal of Investigative Dermatology
Volume128
Issue number2
DOIs
StatePublished - Feb 2008

Fingerprint

Platelet Activating Factor
Gene expression
Radiation
Gene Expression
KB Cells
Genes
Irradiation
Tumor Necrosis Factor-alpha
Cytokines
Messenger RNA
platelet activating factor receptor
Microarrays
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Intercellular Signaling Peptides and Proteins
Skin
Epithelial Cells
RNA
Cell Line

ASJC Scopus subject areas

  • Dermatology

Cite this

Augmentation of UVB radiation-mediated early gene expression by the epidermal platelet-activating factor receptor. / Travers, Jeffrey; Edenberg, Howard; Zhang, Qiwei; Al-Hassani, Mohammed; Yi, Qiaofang; Baskaran, Sulochanadevi; Konger, Raymond.

In: Journal of Investigative Dermatology, Vol. 128, No. 2, 02.2008, p. 455-460.

Research output: Contribution to journalArticle

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