Aurora A activation in mitosis promoted by BuGZ

Yuejia Huang, Teng Li, Stephanie C. Ems-McClung, Claire Walczak, Claude Prigent, Xueliang Zhu, Xuemin Zhang, Yixian Zheng

Research output: Contribution to journalArticle

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Abstract

Protein phase separation or coacervation has emerged as a potential mechanism to regulate biological functions. We have shown that coacervation of a mostly unstructured protein, BuGZ, promotes assembly of spindle and its matrix. BuGZ in the spindle matrix binds and concentrates tubulin to promote microtubule (MT) assembly. It remains unclear, however, whether BuGZ could regulate additional proteins to promote spindle assembly. In this study, we report that BuGZ promotes Aurora A (AurA) activation in vitro. Depletion of BuGZ in cells reduces the amount of phosphorylated AurA on spindle MTs. BuGZ also enhances MCAK phosphorylation. The two zinc fingers in BuGZ directly bind to the kinase domain of AurA, which allows AurA to incorporate into the coacervates formed by BuGZ in vitro. Importantly, mutant BuGZ that disrupts the coacervation activity in vitro fails to promote AurA phosphorylation in Xenopus laevis egg extracts. These results suggest that BuGZ coacervation promotes AurA activation in mitosis.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalJournal of Cell Biology
Volume217
Issue number1
DOIs
StatePublished - Jan 1 2018

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Mitosis
Aurora Kinase A
Phosphorylation
Intrinsically Disordered Proteins
Zinc Fingers
Xenopus laevis
Tubulin
Microtubules
Ovum
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Huang, Y., Li, T., Ems-McClung, S. C., Walczak, C., Prigent, C., Zhu, X., ... Zheng, Y. (2018). Aurora A activation in mitosis promoted by BuGZ. Journal of Cell Biology, 217(1), 107-116. https://doi.org/10.1083/jcb.201706103

Aurora A activation in mitosis promoted by BuGZ. / Huang, Yuejia; Li, Teng; Ems-McClung, Stephanie C.; Walczak, Claire; Prigent, Claude; Zhu, Xueliang; Zhang, Xuemin; Zheng, Yixian.

In: Journal of Cell Biology, Vol. 217, No. 1, 01.01.2018, p. 107-116.

Research output: Contribution to journalArticle

Huang, Y, Li, T, Ems-McClung, SC, Walczak, C, Prigent, C, Zhu, X, Zhang, X & Zheng, Y 2018, 'Aurora A activation in mitosis promoted by BuGZ', Journal of Cell Biology, vol. 217, no. 1, pp. 107-116. https://doi.org/10.1083/jcb.201706103
Huang Y, Li T, Ems-McClung SC, Walczak C, Prigent C, Zhu X et al. Aurora A activation in mitosis promoted by BuGZ. Journal of Cell Biology. 2018 Jan 1;217(1):107-116. https://doi.org/10.1083/jcb.201706103
Huang, Yuejia ; Li, Teng ; Ems-McClung, Stephanie C. ; Walczak, Claire ; Prigent, Claude ; Zhu, Xueliang ; Zhang, Xuemin ; Zheng, Yixian. / Aurora A activation in mitosis promoted by BuGZ. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 1. pp. 107-116.
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