Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity

Xin Zhang, Stephanie C. Ems-McClung, Claire Walczak

Research output: Contribution to journalArticle

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Abstract

During mitosis, mitotic centromere-associated kinesin (MCAK) localizes to chromatin/kinetochores, a cytoplasmic pool, and spindle poles. Its localization and activity in the chromatin region are regulated by Aurora B kinase; however, how the cytoplasmic- and pole-localized MCAK are regulated is currently not clear. In this study, we used Xenopus egg extracts to form spindles in the absence of chromatin and centrosomes and found that MCAK localization and activity are tightly regulated by Aurora A. This regulation is important to focus microtubules at aster centers and to facilitate the transition from asters to bipolar spindles. In particular, we found that MCAK colocalized with NuMA and XMAP215 at the center of Ran asters where its activity is regulated by Aurora A-dependent phosphorylation of S196, which contributes to proper pole focusing. In addition, we found that MCAK localization at spindle poles was regulated through another Aurora A phosphorylation site (S719), which positively enhances bipolar spindle formation. This is the first study that clearly defines a role for MCAK at the spindle poles as well as identifies another key Aurora A substrate that contributes to spindle bipolarity.

Original languageEnglish
Pages (from-to)2752-2765
Number of pages14
JournalMolecular Biology of the Cell
Volume19
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Kinesin
Centromere
Spindle Poles
Chromatin
Aurora Kinase B
Phosphorylation
Kinetochores
Centrosome
Xenopus
Mitosis
Microtubules
Ovum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity. / Zhang, Xin; Ems-McClung, Stephanie C.; Walczak, Claire.

In: Molecular Biology of the Cell, Vol. 19, No. 7, 07.2008, p. 2752-2765.

Research output: Contribution to journalArticle

Zhang, Xin ; Ems-McClung, Stephanie C. ; Walczak, Claire. / Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 7. pp. 2752-2765.
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