Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins

Constance E. Brinckerhoff, Teresa I. Mitchell, Michael J. Karmilowicz, Barbara Kluve-Beckerman, Merrill Benson

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Two autocrine proteins of 14 and 12 kilodaltons that induce the synthesis of rabbit fibroblast collagenase were identified. The proteins were purified from serum-free culture medium taken from rabbit synovial fibroblasts stimulated with phorbol myristate acetate. The amino-terminal sequences of the 14- and 12-kilodalton species were ∼60 to 80 percent homologous with serum amyloid A and β2 microglobulin, respectively. The polyacrylamide gel-eluted proteins retained the ability to induce collagenase synthesis in rabbit and human fibroblasts. These autocrine proteins may provide a means to modulate collagenase synthesis in normal remodeling as well as in inflammation and disease states.

Original languageEnglish (US)
Pages (from-to)655-657
Number of pages3
JournalScience
Volume243
Issue number4891
StatePublished - 1989

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Serum Amyloid A Protein
Collagenases
Rabbits
Proteins
Fibroblasts
Matrix Metalloproteinase 8
Serum-Free Culture Media
Tetradecanoylphorbol Acetate
Inflammation

ASJC Scopus subject areas

  • General

Cite this

Brinckerhoff, C. E., Mitchell, T. I., Karmilowicz, M. J., Kluve-Beckerman, B., & Benson, M. (1989). Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins. Science, 243(4891), 655-657.

Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins. / Brinckerhoff, Constance E.; Mitchell, Teresa I.; Karmilowicz, Michael J.; Kluve-Beckerman, Barbara; Benson, Merrill.

In: Science, Vol. 243, No. 4891, 1989, p. 655-657.

Research output: Contribution to journalArticle

Brinckerhoff, CE, Mitchell, TI, Karmilowicz, MJ, Kluve-Beckerman, B & Benson, M 1989, 'Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins', Science, vol. 243, no. 4891, pp. 655-657.
Brinckerhoff CE, Mitchell TI, Karmilowicz MJ, Kluve-Beckerman B, Benson M. Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins. Science. 1989;243(4891):655-657.
Brinckerhoff, Constance E. ; Mitchell, Teresa I. ; Karmilowicz, Michael J. ; Kluve-Beckerman, Barbara ; Benson, Merrill. / Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins. In: Science. 1989 ; Vol. 243, No. 4891. pp. 655-657.
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