Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins

Constance E. Brinckerhoff, Teresa I. Mitchell, Michael J. Karmilowicz, Barbara Kluve-Beckerman, Merrill D. Benson

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Abstract

Two autocrine proteins of 14 and 12 kilodaltons that induce the synthesis of rabbit fibroblast collagenase were identified. The proteins were purified from serum-free culture medium taken from rabbit synovial fibroblasts stimulated with phorbol myristate acetate. The amino-terminal sequences of the 14- and 12-kilodalton species were ∼60 to 80 percent homologous with serum amyloid A and β2 microglobulin, respectively. The polyacrylamide gel-eluted proteins retained the ability to induce collagenase synthesis in rabbit and human fibroblasts. These autocrine proteins may provide a means to modulate collagenase synthesis in normal remodeling as well as in inflammation and disease states.

Original languageEnglish (US)
Pages (from-to)655-657
Number of pages3
JournalScience
Volume243
Issue number4891
DOIs
StatePublished - Jan 1 1989

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Brinckerhoff, C. E., Mitchell, T. I., Karmilowicz, M. J., Kluve-Beckerman, B., & Benson, M. D. (1989). Autocrine induction of collagenase by serum amyloid A-like and β2-microglobulin-like proteins. Science, 243(4891), 655-657. https://doi.org/10.1126/science.2536953